The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice -: art. no. 14

被引:53
作者
Chiavolini, D
Memmi, G
Maggi, T
Iannelli, F
Pozzi, G
Oggioni, MR
机构
[1] Dipartimento di Biologia Molecolare, Lab. di Microbiol. Molec./Biotecnol., Università di Siena, Siena
关键词
D O I
10.1186/1471-2180-3-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MFI outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival. Results: Observation of survival percentages over time and detection of LD(50)s of knock out mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice. Conclusions: These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease.
引用
收藏
页码:1 / 9
页数:9
相关论文
共 38 条
[1]   The puzzle of zmpB and extensive chain formation, autolysis defect and non-translocation of choline-binding proteins in Streptococcus pneumoniae [J].
Bergé, M ;
García, P ;
Iannelli, F ;
Prère, MF ;
Granadel, C ;
Polissi, A ;
Claverys, JP .
MOLECULAR MICROBIOLOGY, 2001, 39 (06) :1651-1660
[2]  
BLUE CE, 2002, 6 EUR M MOL BIOL PNE
[3]   Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae [J].
Bricker, AL ;
Camilli, A .
FEMS MICROBIOLOGY LETTERS, 1999, 172 (02) :131-135
[4]   THE ROLE OF PNEUMOLYSIN AND AUTOLYSIN IN THE PATHOLOGY OF PNEUMONIA AND SEPTICEMIA IN MICE INFECTED WITH A TYPE-2 PNEUMOCOCCUS [J].
CANVIN, JR ;
MARVIN, AP ;
SIVAKUMARAN, M ;
PATON, JC ;
BOULNOIS, GJ ;
ANDREW, PW ;
MITCHELL, TJ .
JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (01) :119-123
[5]   CONSTRUCTION AND EVALUATION OF NEW DRUG-RESISTANCE CASSETTES FOR GENE DISRUPTION MUTAGENESIS IN STREPTOCOCCUS-PNEUMONIAE, USING AN AMI TEST PLATFORM [J].
CLAVERYS, JP ;
DINTILHAC, A ;
PESTOVA, EV ;
MARTIN, B ;
MORRISON, DA .
GENE, 1995, 164 (01) :123-128
[6]   Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate [J].
Dopazo, J ;
Mendoza, A ;
Herrero, J ;
Caldara, F ;
Humbert, Y ;
Friedl, L ;
Guerrier, M ;
Grand-Schenk, E ;
Gandin, C ;
De Franceso, M ;
Polissi, A ;
Buell, G ;
Feger, G ;
García, E ;
Peitsch, M ;
García-Bustos, JF .
MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 2001, 7 (02) :99-125
[7]   The contribution of accessory toxins of Vibrio cholerae O1 El Tor to the proinflammatory response in a murine pulmonary cholera model [J].
Fullner, KJ ;
Boucher, JC ;
Hanes, MA ;
Haines, GK ;
Meehan, BM ;
Walchle, C ;
Sansonetti, PJ ;
Mekalanos, JJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 2002, 195 (11) :1455-1462
[9]   Role of genetic resistance in invasive pneumococcal infection: Identification and study of susceptibility and resistance in inbred mouse strains [J].
Gingles, NA ;
Alexander, JE ;
Kadioglu, A ;
Andrew, PW ;
Kerr, A ;
Mitchell, TJ ;
Hopes, E ;
Denny, P ;
Brown, S ;
Jones, HB ;
Little, S ;
Booth, GC ;
McPheat, WL .
INFECTION AND IMMUNITY, 2001, 69 (01) :426-434
[10]   Mosaic genes and mosaic chromosomes:: Intra- and interspecies genomic variation of Streptococcus pneumoniae [J].
Hakenbeck, R ;
Balmelle, N ;
Weber, B ;
Gardès, C ;
Keck, W ;
de Saizieu, A .
INFECTION AND IMMUNITY, 2001, 69 (04) :2477-2486