The yeast Saccharomyces cerevisiae stress response protein Hsp12p decreases the gel strength of agarose used as a model system for the β-glucan layer of the cell wall

被引：13

作者：

Karreman, RJ ^{[1
]}

Brandt, WF ^{[1
]}

Lindsey, GG ^{[1
]}

机构：

[1] Univ Cape Town, Dept Mol & Cellular Biol, ZA-7701 Rondebosch, South Africa

来源：

CARBOHYDRATE POLYMERS | 2005年 / 60卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

rheometry; gelation temperature; turbidity;

D O I：

10.1016/j.carbpol.2004.12.004

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Incorporation of the cell wall LEA-like stress response protein Hsp12p from the yeast Saccharomyces cerevisiae into the carbohydrate polymer agarose was found to reduce the gel strength of the agarose matrix. Solutes known to stimulate Hsp12p production in yeast were found to increase the gelation temperature, the turbidity and the gel strength of the agarose matrix. Increased gel strength brought about by solute incorporation was found to be reversed by co-incorporation of Hsp12p or by the chaotropic solute urea. These results suggested a mode of action for Hsp12p, assuming that agarose is a reasonable macroscopic model system for investigating the effects of cell wall proteins on the properties of the yeast cell wall. (c) 2005 Published by Elsevier Ltd.

引用

页码：193 / 198

页数：6

共 19 条

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