Enhanced Pseudomonas aeruginosa biofilm development mediated by human neutrophils

被引:199
作者
Walker, TS
Tomlin, KL
Worthen, GS
Poch, KR
Lieber, JG
Saavedra, MT
Fessler, MB
Malcolm, KC
Vasil, ML
Nick, JA
机构
[1] Natl Jewish Med & Res Ctr, Dept Med, Denver, CO 80206 USA
[2] Univ Colorado, Sch Med, Div Pulm Sci & Crit Care Med, Denver, CO 80262 USA
[3] Univ Colorado, Sch Med, Dept Microbiol & Immunol, Denver, CO 80262 USA
关键词
D O I
10.1128/IAI.73.6.3693-3701.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cystic fibrosis (CF) lung disease features persistent neutrophil accumulation to the airways from the time of infancy. CF children are frequently exposed to Pseudomonas aeruginosa, and by adulthood, 80% of CF patients are chronically infected. The formation of biofilms is a particularly important phenotypic characteristic of P. aeruginosa that allows for bacterial survival despite aggressive antibiotic therapy and an exuberant immune response. Here, we show that the presence of neutrophils enhances initial P. aeruginosa biofilm development over a period of 72 h through the formation of polymers comprised of actin and DNA. F-actin was found to be a site of attachment for P. aeruginosa. These actin and DNA polymers are present in CF sputum, and disruption of the polymers dispersed the associated P. aeruginosa cells and reduced biofilm development. These findings demonstrate a potential maladaptation of the primary innate response. When the host fails to eradicate the infection, cellular components from necrotic neutrophils can serve as a biological matrix to facilitate P. aeruginosa biofilm formation.
引用
收藏
页码:3693 / 3701
页数:9
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