Production and crystallization of MHC class I B allele single peptide complexes

被引：29

作者：

Reid, SW

Smith, KJ

Jakobsen, BK

OCallaghan, CA

Reyburn, H

Harlos, K

Stuart, DI

McMichael, AJ

Bell, JI

Jones, EY

机构：

[1] LAB MOLEC BIOPHYS,OXFORD OX1 3QU,ENGLAND

[2] JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN MED,INST MOLEC MED,MOLEC IMMUNOL GRP,OXFORD OX3 9DU,ENGLAND

[3] OXFORD CTR MOLEC SCI,OXFORD OX1 3QT,ENGLAND

来源：

FEBS LETTERS | 1996年 / 383卷 / 1-2期

关键词：

protein expression; MHC class I; HLA B allele; X-ray crystallography; peptide complex;

D O I：

10.1016/0014-5793(96)00226-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P2(1)2(1)2(1), with similar unit cell dimensions of approximately 52 Angstrom X 81 Angstrom x 112 Angstrom, contain one complex per asymmetric unit and diffract to approximately 2.0 Angstrom resolution.

引用

收藏

页码：119 / 123

页数：5

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