Facilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening

被引：108

作者：

Khersonsky, SM

Jung, DW

Kang, TW

Walsh, DP

Moon, HS

Jo, H

Jacobson, EM

Shetty, V

Neubert, TA

Chang, YT ^{[1
]}

机构：

[1] NYU, Dept Chem, New York, NY 10003 USA

[2] MIT, Whitehead Inst, Cambridge, MA 02142 USA

[3] NYU, Sch Med, Dev Genet Program, Dept Cell Biol, New York, NY 10016 USA

[4] NYU, Sch Med, Dept Pharmacol, Skirball Inst Biomol Med, New York, NY 10016 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 39期

关键词：

D O I：

10.1021/ja035334d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An improved forward chemical genetics approach was successfully demonstrated using a tagged library concept. A small-molecule triazine library with linkers was used to screen for brain/eye developmental phenotypes in a zebrafish embryo system. This approach enabled the rapid isolation of the target proteins by facile affinity matrix preparation and elucidated the first small-molecule inhibitors for several ribosomal accessory proteins or their complex as the target. Copyright © 2003 American Chemical Society.

引用

页码：11804 / 11805

页数：2

共 24 条

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