Prenatal evaluation of kidney function in mice using dynamic contrast-enhanced magnetic resonance imaging

被引:13
作者
Chapon, C
Franconi, F
Roux, J
Le Jeune, JJ
Lemaire, L
机构
[1] Univ Angers, INSERM, Ingn Vectorisat U646, F-49100 Angers, France
[2] Univ Angers, Serv Commun Anal Spect, F-49100 Angers, France
[3] Univ Angers, Serv Commun Anim Hosp Univ, F-49100 Angers, France
来源
ANATOMY AND EMBRYOLOGY | 2005年 / 209卷 / 04期
关键词
in utero functional imaging; MRI; contrast agent; gadolinium; embryo; kidney;
D O I
10.1007/s00429-004-0451-9
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Glomerular differentiation starts as soon as embryonic stage 12 in mice and suggests that kidneys may be functional at this stage. Dynamic contrast-enhanced magnetic resonance microscopy, a noninvasive imaging technique, was used to assess renal function establishment in utero. Indeed, in adults ( n= 3), an intravenous injection of gadolinium-DOTA induced in a first step a massive and rapid drop in kidney signal intensity followed, in a second step, by a drop in bladder signal intensity. The delay in signal changes between kidney and bladder reflected glomerular filtration. Pregnant mice underwent anatomical and dynamic contrast-enhanced magnetic resonance microscopy on postcoital days 12 - 13 ( n= 2), 13 - 14 ( n= 1), 14 - 15 ( n= 3), 15 - 16 ( n= 2), 16 - 17 ( n= 3), 17 - 18 ( n= 3), and 18 - 19 ( n= 1). Kidneys and bladder were unambiguously depicted prior to contrast agent injection on stage 15 - 16 embryos. Contrast agent injection allowed kidney, detection as early as stage 12 - 13 but not bladder. Kinetics of signal changes demonstrated that glomerular filtration is established at embryonic stage 15 - 16 in mice. Thus, anatomical and dynamic contrast-enhanced magnetic resonance microscopy may be a powerful noninvasive method for in vivo prenatal developmental and functional studies.
引用
收藏
页码:263 / 267
页数:5
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