Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids

被引:59
作者
Boussaad, S
Dziri, L
Arechabaleta, R
Tao, NJ [1 ]
Leblanc, RM
机构
[1] Florida Int Univ, Dept Phys, Miami, FL 33199 USA
[2] Univ Miami, Dept Chem, Coral Gables, FL 33124 USA
关键词
D O I
10.1021/la980319l
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The structural and electron-transfer properties of cytochrome c (Cyt c) Langmuir-Blodgett (LB) films have been studied on graphite electrode with tapping mode atomic force microscopy and cyclic voltammetry (CV). Cyt c in the LB films forms an ordered monolayer in which the individual proteins pack into a quasi-hexagonal structure. The monolayer undergoes a reversible electron-transfer reaction in phosphate buffer. The interactions of Cyt c with cardiolipin (CL) and phosphatidylcholine (PC) LB films have been studied. The LB films of CL and PC are both ordered on graphite, but their interactions with Cyt c are quite different. On a CL monolayer, Cyt c adsorbs spontaneously and the adsorbed protein preserves the electron-transfer reaction. However, on a PC monolayer, Cyt c does not adsorb.
引用
收藏
页码:6215 / 6219
页数:5
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