Determination of six functional compounds in Crataegus pinatifida BGE by capillary zone electrophoresis

The method of capillary zone electrophoresis was developed to separate four pharmacologically active flavones, one flavonoid, and cholorgenic acid in Crataegus. Experiments showed that the baseline separation of six compounds were finished within 15 min, with an optimized buffer system containing 40 mmol/L borax and 10% (V/V) acetonitrile, and being adjusted to pH 8.20 by 90% (V/V) H3PO4. The relative standard deviation of the concentration of analytes in samples varied in the range of 2.73-6.52% and the recovery of spiked sample within 94.25-98.23% (five replicates), and detection limits were below 0.55 mg/L for each analyte. The distribution of the six active compounds was found to differ quantitatively in different parts of Crataegus. Vitexin-2"-O-rhamnose was the most flavone in leaves, but in fruit epicatechin was very high and hyperside was not detected in this species of Crataegus. There were very little active compounds in flowers.