Simultaneous detection of NO and ROS by ESR in biological systems

被引：29

作者：

Cao, YL ^{[1
]}

Guo, P ^{[1
]}

Xu, YC ^{[1
]}

Zhao, BL ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China

来源：

NITRIC OXIDE, PT E | 2005年 / 396卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/S0076-6879(05)96008-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A large body of evidence shows that the generation of nitric oxide (NO) and reactive oxygen species (ROS) and the rate of ROS/NO play an important role in the biological system. We developed a method to simultaneously detect NO free radical and ROS in biological systems using ERS spin trapping technique. The adduct (DETC)(2)-Fe(2+)-NO and N-tert-butyl-alpha-phenylnitrone (PBN)-ROS in biological systems can be extracted by organic solvent and then measured on an electron spin resonance (ESR) spectrometer at room temperature because the g = 2.035 of (DETC)(2)-Fe(2+)-NO is different from that of PBN-ROS (g = 2.005) and their ESR signals can be separated clearly. Using this method, we measured the production of NO and ROS in plant and animal systems.

引用

页码：77 / 83

页数：7

共 10 条

[1] Role of active oxygen species and NO in plant defence responses [J].

Bolwell, GP .

CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (04) :287-294

[2]

Cao YL, 2002, FREE RADICAL BIO MED, V33, pS74

[3] Changes in reactive oxygen species (ROS) production in rat brain during global perinatal asphyxia: an ESR study [J].

Capani, F ;

Loidl, CF ;

Aguirre, F ;

Piehl, L ;

Facorro, G ;

Hager, A ;

De Paoli, T ;

Farach, H ;

Pecci-Saavedra, J .

BRAIN RESEARCH, 2001, 914 (1-2) :204-207

[4] Identification of a plant nitric oxide synthase gene involved in hormonal signaling [J].

Guo, FQ ;

Okamoto, M ;

Crawford, NM .

SCIENCE, 2003, 302 (5642) :100-103

[5] ONLINE DETECTION OF NITRIC-OXIDE FORMATION IN LIQUID AQUEOUS PHASE BY ELECTRON-PARAMAGNETIC RESONANCE SPECTROSCOPY [J].

MORDVINTCEV, P ;

MULSCH, A ;

BUSSE, R ;

VANIN, A .

ANALYTICAL BIOCHEMISTRY, 1991, 199 (01) :142-146

[6] NITRIC-OXIDE AS A SECRETORY PRODUCT OF MAMMALIAN-CELLS [J].

NATHAN, C .

FASEB JOURNAL, 1992, 6 (12) :3051-3064

[7] Improved method to detect nitric oxide in biological systems [J].

Zhang, D ;

Xiong, J ;

Hu, J ;

Li, Y ;

Zhao, B .

APPLIED MAGNETIC RESONANCE, 2001, 20 (03) :345-356

[8] Developmental expression and activity variation of nitric oxide synthase in the brain of golden hamster [J].

Zhang, YT ;

Zhang, DL ;

Cao, YL ;

Zhao, BL .

BRAIN RESEARCH BULLETIN, 2002, 58 (04) :385-389

[9] RIGOROUS STUDY OF CHARGED CARRIERS IN ELECTRIC-FIELDS WITH ALTERNATING SITE ENERGIES AND INTERSITE INTERACTIONS [J].

ZHAO, XG .

COMMUNICATIONS IN THEORETICAL PHYSICS, 1993, 20 (04) :409-414

[10] Detection of nitric oxide in tissue by spin trapping EPR spectroscopy and triacetylglycerol extraction [J].

Zhou, GY ;

Zhao, BL ;

Hou, JW ;

Li, MF ;

Chen, C ;

Xin, WJ .

BIOTECHNOLOGY TECHNIQUES, 1999, 13 (08) :507-511

← 1 →