In vitro enzymatic degradation of nanoparticles prepared from hydrophobically-modified poly(γ-glutamic acid)

被引：53

作者：

Akagi, T

Higashi, M

Kaneko, T

Kida, T

Akashi, M

机构：

[1] Osaka Univ, Dept Appl Chem, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] CREST, JST, Tokyo, Japan

[3] Kagoshima Univ, Grad Sch Sci & Engn, Dept Nanostruct & Adv Mat, Kagoshima 8900065, Japan

来源：

MACROMOLECULAR BIOSCIENCE | 2005年 / 5卷 / 07期

关键词：

amphiphiles; biodegradable; drug delivery systems; enzymes; nanoparticles;

D O I：

10.1002/mabi.200500036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amphiphilic poly(gamma-glutamic acid) (gamma-PGA) was prepared by the introduction Of L-phenylalanine ethylester (L-PAE) as a side chain. This gamma-PGA-graft-L-PAE formed monodispersed nanoparticles in water. The particle size of the gamma-PGA nanoparticles could be controlled by the degree of L-PAE grafting. The hydrolytic degradation and enzymatic degradation by gamma-glutamyl transpeptidase (gamma-GTP) of these gamma-PGA nanoparticles was studied by gel permeation chromatography (GPC) and scanning electron microscopy (SEM). The hydrolysis ratio of gamma-PGA was found to decrease upon increasing the hydrophilicity of the gamma-PGA. The degradation of the gamma-PGA backbone by gamma-GTP resulted in a dramatic change in nanoparticle morphology. With increasing time, the gamma-PGA nanoparticles reduced in size and finally disappeared completely.

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收藏

页码：598 / 602

页数：5

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