Studies on quantum dots synthesized in aqueous solution for biological labeling via electrostatic interaction

被引：75

作者：

Lin, ZB

Cui, SX

Zhang, H

Chen, QD

Yang, B

Su, XG

Zhang, JH

Jin, QH ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Inst Analyt Sci, Changchun 130023, Peoples R China

[2] Jilin Univ, Coll Chem, Key Lab Supramol Struct & Mat, Changchun 130023, Peoples R China

[3] Chinese Acad Sci, Lab Excited State Proc, Changchun 130021, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2003年 / 319卷 / 02期

关键词：

water-soluble; quantum dots; papain; electrostatic interaction; conjugate;

D O I：

10.1016/S0003-2697(03)00287-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

3-Mercaptopropyl acid-stabilized CdTe nanoparticles synthesized in aqueous solution are effectively bound to a biomacro-molecule, papain, via electrostatic interaction. The conjugation between the nanoparticles and the papain is demonstrated by UV-Vis absorption, photoluminescence spectroscopy, transmission electron microscopy, and fluorescence micrographs. The biological activity of papain is maintained after the conjugation. The effects of the quantity of papain and the size of nanoparticles on the fluorescence characteristics of the CdTe-papain bioconjugates were studied. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：239 / 243

页数：5

共 21 条

[1] Semiconductor nanocrystals as fluorescent biological labels [J].