Hyperpolarization-activated (Ih) current in mouse vestibular primary neurons

被引：20

作者：

Chabbert, C ^{[1
]}

Chambard, JM ^{[1
]}

Valmier, J ^{[1
]}

Sans, A ^{[1
]}

Desmadryl, G ^{[1
]}

机构：

[1] INSERM, U432, F-34095 Montpellier 5, France

来源：

NEUROREPORT | 2001年 / 12卷 / 12期

关键词：

current; and voltage-clamp; Cs+; I-h; vestibular primary neurons; ZD7288;

D O I：

10.1097/00001756-200108280-00022

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

The presence of a hyperpolarization-activated inward current (I-h) was investigated in mouse vestibular primary neurons using the whole-cell patch-clamp technique. In current-clamp configuration, injection of hyperpolarizing currents induced variations of membrane voltage with prominent time-dependent rectification increasing with current amplitudes. This effect was abolished by 2 mM Cs+ or 100 muM ZD7288. In voltage-clamp configuration, hyperpolarization pulses from -60 mV to -140 mV triggered a slow activating and non inactivating inward current that was sensitive to the two blockers, but insensitive to SmM Ba2+. Changing Na+ and K+ concentrations demonstrated that Ih current is carried by both these monovalent cations. This is the first demonstration of a Ih current in vestibular primary neurons. NeuroReport 12:2701-2704 (C) 2001 Lippincott Williams & Wilkins.

引用

页码：2701 / 2704

页数：4

共 12 条

[1]

Hyperpolarization-activated, mixed-cation current (Ih) in octopus cells of the mammalian cochlear nucleus [J].