Cell-free fusion of bacteria-containing phagosomes with endocytic compartments

被引:38
作者
Becken, Ulrike [1 ]
Jeschke, Andreas [1 ]
Veltman, Katharina [1 ]
Haas, Albert [1 ]
机构
[1] Univ Bonn, Inst Cell Biol, D-53121 Bonn, Germany
关键词
latex beads; phagocytosis; macrophage; phagolysosome; vacuole; MEMBRANE-FUSION; LATE ENDOSOMES; SALMONELLA-TYPHIMURIUM; LYSOSOME FUSION; SNARE COMPLEXES; HOMOTYPIC FUSION; RAB PROTEINS; TRAFFICKING; TRANSPORT; MACROPHAGES;
D O I
10.1073/pnas.1007295107
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Uptake of microorganisms by professional phagocytic cells leads to formation of a new subcellular compartment, the phagosome, which matures by sequential fusion with early and late endocytic compartments, resulting in oxidative and nonoxidative killing of the enclosed microbe. Few tools are available to study membrane fusion between phagocytic and late endocytic compartments in general and with pathogen-containing phagosomes in particular. We have developed and applied a fluorescence microscopy assay to study fusion of microbe-containing phagosomes with different-aged endocytic compartments in vitro. This revealed that fusion of phagosomes containing nonpathogenic Escherichia coli with lysosomes requires Rab7 and SNARE proteins but not organelle acidification. In vitro fusion experiments with phagosomes containing pathogenic Salmonella enterica serovar Typhimurium indicated that reduced fusion of these phagosomes with early and late endocytic compartments was independent of endosome and cytosol sources and, hence, a consequence of altered phagosome quality.
引用
收藏
页码:20726 / 20731
页数:6
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