Proliferative feeder cells support prolonged expansion of human embryonic stem cells

被引：8

作者：

Xie, CQ ^{[1
]}

Lin, G ^{[1
]}

Yuan, D ^{[1
]}

Wang, J ^{[1
]}

Liu, TC ^{[1
]}

Lu, GX ^{[1
]}

机构：

[1] Cent S Univ, Inst Human Reprod & Stem Cell Engn, Natl Ctr Human Stem Cell Res & Engn, Changsha 410078, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2005年 / 29卷 / 08期

关键词：

human embryonic stem cells; self-renewal; feeder; proliferative;

D O I：

10.1016/j.cellbi.2005.03.015

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Long-term proliferation of human embryonic stem (hES) cells is currently achieved by co-culturing with mitotically inactive primary mouse embryonic fibroblasts (mEFs), which serve as feeder cells. This study explores the possibility that proliferative mEFs can be used as feeder cells to maintain the prolonged expansion of hES cells. All undifferentiated hES cell clumps were re-plated on six different densities of proliferative mEFs. hES colonies cultured on 1 X 10(5)-5 X 10(5) proliferative mEFs amplified over 130 days of continuous culture and remained undifferentiated, as did those cultured on mitotically inactive mEFs. This suggests that certain densities of proliferative mEFs can maintain the propagation of hES cells, which may be helpful for identifying the cytokines and adhesion molecules that are required for their self-renewal. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：623 / 628

页数：6

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