DyeCycle violet used for side population detection is a substrate of P-glycoprotein

被引：26

作者：

Boesch, Maximilian ^{[2
]}

Reimer, Daniel ^{[3
]}

Rumpold, Holger ^{[4
]}

Zeimet, Alain G. ^{[3
]}

Sopper, Sieghart ^{[2
,5
]}

Wolf, Dominik ^{[1
,2
]}

机构：

[1] Univ Hosp Bonn, Dept Hematol & Oncol, D-53111 Bonn, Germany

[2] Innsbruck Med Univ, Tyrolean Canc Res Inst, Innsbruck, Austria

[3] Innsbruck Med Univ, Dept Gynecol & Obstet, Innsbruck, Austria

[4] Klinikum Barmherzigen Schwestern Linz, Linz, Austria

[5] Innsbruck Med Univ, FACS Core Facil, Innsbruck, Austria

来源：

CYTOMETRY PART A | 2012年 / 81A卷 / 06期

基金：

奥地利科学基金会;

关键词：

cancer stem cells; side population sorting; DyeCycle Violet; ABC drug transporter; P-glycoprotein; MULTIDRUG RESISTANCE GENE; HEMATOPOIETIC STEM-CELLS; CANCER-CELLS; LUNG-CANCER; IDENTIFICATION; TRANSPORTER; EXPRESSION; MDR1; HOECHST-33342; PROGENITORS;

D O I：

10.1002/cyto.a.22038

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Cancer stem cells (CSC) are increasingly recognized as key target cells for cancer therapy because they are both tumorigenic and chemoresistant and, therefore, can give rise to recurrent disease. Side population (SP) sorting using an ultraviolet (UV) laser is an established method to isolate CSC based on ABC drug transporter-dependent (e.g., ABCG2) Hoechst 33342 efflux. We here show that Vybrant (R) DyeCycle (TM) Violet (DCV), a DNA-binding fluorophor allowing SP sorting using a non-UV laser (such as violet laser), is transported via P-glycoprotein (PgP). Because PgP might be particularly abundant in multidrug-resistant cancer cells rather than bona fide CSC, investigators using DCV should be aware that this strategy might also detect PgP-expressing non-CSC. (c) 2012 International Society for Advancement of Cytometry

引用

页码：517 / 522

页数：6

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