Effect of Tris-HCl Buffer on DNA Adsorption by a Variety of Soil Constituents

被引：24

作者：

Saeki, Kazutoshi ^{[3
]}

Kunito, Takashi ^{[2
]}

Sakai, Masao ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Agr, Kagoshima 8900065, Japan

[2] Shinshu Univ, Fac Sci, Matsumoto, Nagano 3908621, Japan

[3] Kyushu Univ, Biotron Inst, Higashi Ku, Fukuoka 8128581, Japan

来源：

MICROBES AND ENVIRONMENTS | 2011年 / 26卷 / 01期

基金：

日本学术振兴会;

关键词：

DNA adsorption; Tris-HCl buffer; allophane; soil; montmorillonite; BACILLUS-SUBTILIS; CLAY-MINERALS; COLLOIDAL PARTICLES; HUMIC SUBSTANCES; TRANSFORMATION; MONTMORILLONITE; DEGRADATION; KAOLINITE; CONSTANT; SILICA;

D O I：

10.1264/jsme2.ME10172

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated the effect of tris(hydroxymethyl) aminomethane hydrochloride (Tris-HCl) buffer (pH 7.0) as a bulk solution on the adsorption of DNA by gibbsite, goethite, montmorillonite, kaolinite, synthetic and natural allophanes, two humic acids and two andosols. The natural allophane, gibbsite, kaolinite and an andosol adsorbed significantly more DNA in a 0.1 M Tris-HCl buffer than in a 0.1 M NaCl solution (t-test, P < 0.005). In contrast, montmorillonite adsorbed significantly less DNA in the Tris-HCl than NaCl solution (P < 0.05). Care should be taken when using Tris-HCl in studies on the adsorption of extracellular DNA molecules by soil particles.

引用

页码：88 / 91

页数：4

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