Mapping the porcine RN gene to chromosome 15

Microsatellite markers have been used to map the porcine RN gene to chromosome 15 as previously reported. This locus affects the technological quality of meat in pigs. Resource families were established by mating 16 crossbred boars (Hampshire x Pietrain) to 65 crossbred sows (Large White x Landrace). From 564 progeny meat samples were taken from the musculus longissimus dorsi 24 h post mortem and the glycogen concentration was determined. The assessment of RN genotypes is based on the measurement of the glycogen concentration. Boars were heterozygous (RN(-)/rn+) and sows were homozygous (rn(+)/rn(+)) at the RN locus. A subsample of 263 animals was typed with four microsatellite markers from chromosome 15. Linkage analysis confirmed that the RN locus maps to chromosome 15, and showed linkage to Sw936 at a distance of 4 cM.