How not to search for isochores: A reply to Cohen et al.

被引：14

作者：

Clay, O ^{[1
]}

Bernardi, G ^{[1
]}

机构：

[1] Staz Zool Anton Dohrn, Lab Mol Evolut, I-80121 Naples, Italy

来源：

MOLECULAR BIOLOGY AND EVOLUTION | 2005年 / 22卷 / 12期

关键词：

base composition; evolution; heterogeneity; long-range correlations;

D O I：

10.1093/molbev/msi231

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a recent paper in these pages, Cohen et al. search for isochores in the human genome, based on a system of attributes that they assign to isochores. The putative isochores that they find and choose for presentation are almost all below 45% GC and cover only about 41% of the genome. Closer inspection reveals that the authors' methodology systematically loses GC-rich isochores because it does not anticipate the considerable fluctuations and corresponding long-range correlations that characterize mammalian DNA and that are highest in GC-rich DNA. Thus, they over-fragment GC-rich isochores (and also many GC-poor isochores) beyond recognition.

引用

页码：2315 / 2317

页数：3