Bacterial Sunscreen: Layer-by-Layer Deposition of UV-Absorbing Polymers on Whole-Cell Biosensors

被引：23

作者：

Eby, D. Matthew ^{[1
,2
]}

Harbaugh, Svetlana ^{[3
]}

Tatum, Randi N. ^{[1
,2
]}

Farrington, Karen E. ^{[1
,2
]}

Kelley-Loughnane, Nancy ^{[3
]}

Johnson, Glenn R. ^{[2
]}

机构：

[1] Universal Technol Corp, Tyndall AFB, FL 32403 USA

[2] USAF, Res Lab, Mat & Mfg Directorate, Tyndall AFB, FL 32403 USA

[3] USAF, Res Lab, Human Effectiveness Directorate, Wright Patterson AFB, OH 45433 USA

来源：

LANGMUIR | 2012年 / 28卷 / 28期

关键词：

RIBOSWITCH; ENCAPSULATION; MICROBES; SUNLIGHT;

D O I：

10.1021/la3014514

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

UV-protective coatings on live bacterial cells were created from the assembly of cationic and UV-absorbing anionic polyelectrolytes using layer-by-layer (LbL) methodology. A cationic polymer (polyallylamine) and three different anionic polymers with varying absorbance in the UV range (poly(vinyl sulfate), poly(4-styrenesulfonic acid), and humic acid) were used to encapsulate Escherichia coli cells with two different green fluorescent protein (GFP) expression systems: constitutive expression of a UV-excitable GFP (GFPuv) and regulated expression of the intensely fluorescent GFP from amphioxus (GFPa1) through a theophylline-inducible riboswitch. Riboswitches activate protein expression after specific ligand-RNA binding events. Hence, they operate as a cellular biosensor that will activate reporter protein synthesis after exposure to a ligand target. E. coli cells coated with UV-absorbing polymers demonstrated enhanced protection of GFP stability, metabolic activity, and viability after prolonged exposure to radiation from a germicidal lamp. The results show the effectiveness of LbL coatings to provide UV protection to living cells for biotechnological applications.

引用

页码：10521 / 10527

页数：7

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