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Semi-automated selection of DNA aptamers using magnetic particle handling

被引：33
作者：
Wochner, Aniela
Cech, Birgit
Menger, Marcus
Erdmann, Volker A.
Gloekler, Joern
机构：
[1] RiNA GmbH, D-14195 Berlin, Germany
[2] Free Univ Berlin, D-1000 Berlin, Germany
[3] RiNa Netzwerk RNA Technol GmbH, Berlin, Germany
来源：
BIOTECHNIQUES | 2007年 / 43卷 / 03期
关键词：
IN-VITRO SELECTION; PROBE CAPILLARY-ELECTROPHORESIS; HIV-1 TAT PROTEIN; GROWTH-FACTOR; RNA INHIBITORS; LIGANDS; BINDING; AFFINITY; ADENOSINE; RECOGNITION;
D O I：
10.2144/000112532
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a semi-automatic selection procedure for DNA aptamers. Employing a robotic workstation for magnetic particle handling, this method allows for a fast, reproducible, and parallelized selection of DNA aptamers. The selection protocol is designed to provide and reagents, as well as vide high flexibility and versatility in terms of choice of buffers and reagents, as well as stringency of selection. Using this procedure, we have successfully isolated ligand-specific, high-affinity DNA aptamers.
引用
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页码：344 / +
页数：6
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