Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil

被引：54

作者：

Borges, LGD ^{[1
]}

Dalla Vechia, V ^{[1
]}

Corçao, G ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICBS, Dept Microbiol, BR-90004017 Porto Alegre, RS, Brazil

来源：

FEMS MICROBIOLOGY ECOLOGY | 2003年 / 45卷 / 02期

关键词：

repetitive extragenic palindromic elements-polymerase chain reaction; genetic diversity; genetic characterization; water isolate; Escherichia coli;

D O I：

10.1016/S0168-6496(03)00147-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The scope of this study was to establish the genomic diversity existing between Escherichia coli isolates obtained from water samples retrieved from Arroio Feijo, southern Brazil, using the repetitive extragenic palindromic elements-polymerase chain reaction protocol. Ninety-eight different isolates were identified from samples obtained from five sites. Eleven different clusters presenting identical fingerprinting patterns were detected. The dendrogram contained 28 clusters for 70% similarity cut-off. These clusters enabled the observation of 16 single patterns. The results enabled the observation of genetic diversity between isolates obtained in one sampling site, and those from other sampling sites. Site 5 showed the highest diversity (Shannon Weaver, H'=2.92) and site 3 exhibited the lowest diversity degree (Shannon Weaver, H'=2.16). (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：173 / 180

页数：8

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