The clonality of Staphylococcus aureus nasal carriage

被引:104
作者
Cespedes, C
Saïd-Salim, B
Miller, M
Lo, SH
Kreiswirth, BN
Gordon, RJ
Vavagiakis, P
Klein, RS
Lowy, FD
机构
[1] Columbia Univ Coll Phys & Surg, Dept Med, Div Infect Dis, New York, NY 10032 USA
[2] Columbia Univ Coll Phys & Surg, Dept Pathol, New York, NY 10032 USA
[3] Columbia Univ, Mailman Sch Publ Hlth, Dept Epidemiol, New York, NY USA
[4] Montefiore Med Ctr, Panna Technol, New York, NY USA
[5] Montefiore Med Ctr, Dept Med, Div Infect Dis, New York, NY USA
[6] Montefiore Med Ctr, Dept Epidemiol & Populat Hlth, New York, NY USA
[7] Albert Einstein Coll Med, New York, NY USA
[8] Publ Hlth Res Inst, Newark, NJ USA
关键词
D O I
10.1086/427240
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Nasal carriage of Staphylococcus aureus is often a prelude to infection with the same strain. The prevailing assumption has been that colonized individuals carry a single strain. The present study investigated the frequency of simultaneous nasal carriage of multiple strains of S. aureus. Three bacterial colonies from plated samples from colonized subjects were initially compared by pulsed-field gel electrophoresis. Fourteen of 148 S. aureus-positive samples demonstrated at least a difference of a single band; 7 of these 14 samples contained different strains, and 3 of these 7 also belonged to different accessory gene regulator (agr) types. The remaining 7 samples contained clonally related isolates; 3 of these 7 contained pairs that differed by the presence or absence of the staphylococcal chromosomal cassette mec type IV. A mathematical model that we developed predicted that similar to6.6% of S. aureus-colonized individuals carry >1 strain. The present study demonstrates that carriage of discordant S. aureus strains in individuals with nasal colonization occurs regularly and suggests that the nares are likely sites for horizontal genetic exchange among strains.
引用
收藏
页码:444 / 452
页数:9
相关论文
共 42 条
[31]   Molecular analysis of coagulase-negative Staphylococcus isolates from blood cultures:: Prevalence of genotypic variation and polyclonal bacteremia [J].
Sharma, M ;
Riederer, K ;
Johnson, LB ;
Khatib, R .
CLINICAL INFECTIOUS DISEASES, 2001, 33 (08) :1317-1323
[32]   Evaluation of protein A gene polymorphic region DNA sequencing for typing of Staphylococcus aureus strains [J].
Shopsin, B ;
Gomez, M ;
Montgomery, SO ;
Smith, DH ;
Waddington, M ;
Dodge, DE ;
Bost, DA ;
Riehman, M ;
Naidich, S ;
Kreiswirth, BN .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) :3556-3563
[33]   Resistance rather than virulence selects for the clonal spread of methicillin-resistant Staphylococcus aureus:: Implications for MRSA transmission [J].
Shopsin, B ;
Mathema, B ;
Zhao, X ;
Martinez, J ;
Kornblum, J ;
Kreiswirth, BN .
MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 2000, 6 (03) :239-244
[34]   Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians [J].
Shopsin, B ;
Mathema, B ;
Alcabes, P ;
Said-Salim, B ;
Lina, G ;
Matsuka, A ;
Martinez, J ;
Kreiswirth, BN .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (01) :456-459
[35]   Risk factors for otitis media and carriage of multiple strains of Haemophilus influenzae and Streptococcus pneumoniae [J].
St Sauver, J ;
Marrs, CF ;
Foxman, B ;
Somsel, P ;
Madera, R ;
Gilsdorf, JR .
EMERGING INFECTIOUS DISEASES, 2000, 6 (06) :622-630
[36]   Polyclonal staphylococcal endocarditis caused by genetic variability [J].
Van Eldere, J ;
Peetermans, WE ;
Struelens, M ;
Deplano, A ;
Bobbaers, H .
CLINICAL INFECTIOUS DISEASES, 2000, 31 (01) :24-+
[37]   Population studies of methicillin-resistant and -sensitive Staphylococcus aureus strains reveal a lack of variability in the agrD gene, encoding a staphylococcal autoinducer peptide [J].
van Leeuwen, W ;
van Nieuwenhuizen, W ;
Gijzen, C ;
Verbrugh, H ;
van Belkum, A .
JOURNAL OF BACTERIOLOGY, 2000, 182 (20) :5721-5729
[38]   Follow-up of Staphylococcus aureus nasal carriage after 8 years:: Redefining the persistent carrier state [J].
VandenBergh, MFQ ;
Yzerman, EPF ;
van Belkum, A ;
Boelens, HAM ;
Sijmons, M ;
Verbrugh, HA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) :3133-3140
[39]   Polyclonal Staphylococcus endocarditis [J].
VanWijngaerden, E ;
Peetermans, WE ;
VanLierde, S ;
VanEldere, J .
CLINICAL INFECTIOUS DISEASES, 1997, 25 (01) :69-71
[40]   Nasal carriage as a source of Staphylococcus aureus bacteremia. [J].
von Eiff, C ;
Becker, K ;
Machka, K ;
Stammer, H ;
Peters, G .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 344 (01) :11-16