Photobactin:: a catechol siderophore produced by Photorhabdus luminescens, an entomopathogen mutually associated with Heterorhabditis bacteriophora NC1 nematodes

被引:58
作者
Ciche, TA
Blackburn, M
Carney, JR
Ensign, JC [1 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[2] USDA ARS, Beltsville Agr Res Ctr, Insect Biocontrol Lab, Beltsville, MD 20705 USA
[3] Kosan Biosci Inc, Hayward, CA 94545 USA
关键词
D O I
10.1128/AEM.69.8.4706-4713.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The nematode Heterorhabditis bacteriophora transmits a monoculture of Photorhabdus luminescens bacteria to insect hosts, where it requires the bacteria for efficient insect pathogenicity and as a substrate for growth and reproduction. Siderophore production was implicated as being involved in the symbiosis because an ngrA mutant inadequate for supporting nematode growth and reproduction was also deficient in producing siderophore activity and ngrA is homologous to a siderophore biosynthetic gene, entD. The role of the siderophore in the symbiosis with the nematode was determined by isolating and characterizing a mini-Tn5-induced mutant, NS414, producing no detectable siderophore activity. This mutant, being defective for growth in iron-depleted medium, was normal in supporting nematode growth and reproduction, in transmission by the dauer juvenile nematode, and in insect pathogenicity. The mini-Tn5 transposon was inserted into phbH-, whose protein product is a putative peptidyl carrier protein homologous to the nonribosomal peptide synthetase VibF of Vibrio cholerae. Other putative siderophore biosynthetic and transport genes flanking phbH were characterized. The catecholate siderophore was purified, its structure was determined to be 2-(2,3-dihydroxyphenyl)-5-methyl-4,5-dihydro-oxazole-4-carboxylic acid [4-(2,3-dihydroxybenzoylamino)-butyl]-amide, and it was given the generic name photobactin. Antibiotic activity was detected with purified photobactin, indicating that the siderophore may contribute to antibiosis of the insect cadaver. These results eliminate the lack of siderophore activity as the cause for the inadequacy of the ngrA mutant in supporting nematode growth and reproduction.
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页码:4706 / 4713
页数:8
相关论文
共 55 条
[51]   Cloning and characterization of vuuA, a gene encoding the Vibrio vulnificus ferric vulnibactin receptor [J].
Webster, ACD ;
Litwin, CM .
INFECTION AND IMMUNITY, 2000, 68 (02) :526-534
[52]   A multifunctional ATP-binding cassette transporter system from Vibrio cholerae transports vibriobactin and enterobactin [J].
Wyckoff, EE ;
Valle, AM ;
Smith, SL ;
Payne, SM .
JOURNAL OF BACTERIOLOGY, 1999, 181 (24) :7588-7596
[53]   Cloning of a Vibrio cholerae vibriobactin gene cluster: Identification of genes required for early steps in siderophore biosynthesis [J].
Wyckoff, EE ;
Stoebner, JA ;
Reed, KE ;
Payne, SM .
JOURNAL OF BACTERIOLOGY, 1997, 179 (22) :7055-7062
[54]   SELECTION OF FLUORESCENT PSEUDOMONADS ANTAGONISTIC TO ERWINIA-CAROTOVORA AND SUPPRESSIVE OF POTATO SEED PIECE DECAY [J].
XU, GW ;
GROSS, DC .
PHYTOPATHOLOGY, 1986, 76 (04) :414-422
[55]   BIOSYNTHESIS OF IRON-TRANSPORT COMPOUND ENTEROCHELIN MUTANTS OF ESCHERICHIA-COLI UNALLE TO SYNTHESIZE 2,3-DIHYDROXBENZOATE [J].
YOUNG, IG ;
LANGMAN, L ;
LUKE, RKJ ;
GIBSON, F .
JOURNAL OF BACTERIOLOGY, 1971, 106 (01) :51-&