Tuning the anticancer activity of a novel pro-apoptotic peptide using gold nanoparticle platforms

被引：79

作者：

Akrami, Mohammad ^{[1
,2
]}

Balalaie, Saeed ^{[3
]}

Hosseinkhani, Saman ^{[4
,5
]}

Alipour, Mohsen ^{[5
]}

Salehi, Fahimeh ^{[6
]}

Bahador, Abbas ^{[7
]}

Haririan, Ismaeil ^{[1
,2
,8
]}

机构：

[1] Univ Tehran Med Sci, Fac Pharm, Dept Pharmaceut Biomat, Tehran, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Med Biomat Res Ctr, Tehran, Iran

[3] KN Toosi Univ Technol, Peptide Chem Res Ctr, Tehran, Iran

[4] Tarbiat Modares Univ, Fac Biol Sci, Dept Biochem, Tehran, Iran

[5] Tarbiat Modares Univ, Fac Biol Sci, Dept Nanobiotechnol, Tehran, Iran

[6] Univ Tehran, Dept Biochem, Inst Biochem & Biophys, Tehran, Iran

[7] Univ Tehran Med Sci, Sch Med, Dept Microbiol, Tehran, Iran

[8] Univ Tehran Med Sci, Dept Pharmaceut, POB 14155-6451, Tehran, Iran

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

ANTIMICROBIAL PEPTIDES; SURFACE MODIFICATION; MITOCHONDRIA; NANORODS; DELIVERY; SIZE; BIOCONJUGATION; STABILIZATION; CURVATURE; ANTITUMOR;

D O I：

10.1038/srep31030

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Pro-apoptotic peptides induce intrinsic apoptosis pathway in cancer cells. However, poor cellular penetration of the peptides is often associated with limited therapeutic efficacy. In this report, a series of peptide-gold nanoparticle platforms were developed to evaluate the anticancer activity of a novel alpha-lipoic acid-peptide conjugate, LA-WKRAKLAK, with respect to size and shape of nanoparticles. Gold nanoparticles (AuNPs) were found to enhance cell internalization as well as anticancer activity of the peptide conjugates. The smaller nanospheres showed a higher cytotoxicity, morphological change and cellular uptake compared to larger nanospheres and nanorods, whereas nanorods showed more hemolytic activity compared to nanospheres. The findings suggested that the anticancer and biological effects of the peptides induced by intrinsic apoptotic pathway were tuned by peptide-functionalized gold nanoparticles (P-AuNPs) as a function of their size and shape.

引用

页数：12

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