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Epitope tagging

被引：140
作者：
Jarvik, JW [1 ]
Telmer, CA [1 ]
机构：
[1] Carnegie Mellon Univ, Dept Biol Sci, Pittsburgh, PA 15213 USA
来源：
ANNUAL REVIEW OF GENETICS | 1998年 / 32卷
关键词：
epitope tagging; protein fusion; gene fusion; antibodies; proteomics;
D O I：
10.1146/annurev.genet.32.1.601
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. This review discusses the major advantages and limitations of epitope tagging and describes a number of recent applications. Major areas of application include monitoring protein expression, localizing proteins at the cellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Recently the method has also found use in transgenic and gene therapy studies and in the emerging fields of functional genomics and proteomics.
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页码：601 / 618
页数：18
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