Experimental manipulation of genomic methylation

被引：12

作者：

JacksonGrusby, L ^{[1
]}

Jaenisch, R ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02142

来源：

SEMINARS IN CANCER BIOLOGY | 1996年 / 7卷 / 05期

基金：

美国国家卫生研究院;

关键词：

cancer; cytosine methylation; gene expression; gene targeting; genomic imprinting; genomic instability; mutagenesis;

D O I：

10.1006/scbi.1996.0034

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cytosine methylation is an important mechanism of gene regulation in mammals. Mouse embryos with reduced DNA methylation due to targeted disruption of the DNA methyltransferase gene show deregulated expression of imprinted genes. Loss of imprinting associated with lass of allele-specific methylation is one example of an epigenetic alteration found in tumor cells. Changes in DNA methylation may also be associated with facilitating protooncogene expression and inactivating tumor suppressor genes. However, cytosine methylation has additional deleterious consequences for the genome as well. CpG dinucleotides, the target of DNA methylation, are five-fold underpresented in the genome due to the high mutability of methylated cytosine. C-T transition mutations resulting from deamination of 5-methylcytosine are involved in both genetic disease and cancer. Lastly, aberrant DNA methylation may promote the genetic instability of a chromosomal locus. We review the genetic and epigenetic roles for DNA methylation during tumorigenesis gleaned from altered methycytosine patterns in tumor cells, and from pharmacologic, dietary or genetic manipulation of DNA methylation levels.

引用

页码：261 / 268

页数：8

共 85 条

[11] DEREGULATION OF BOTH IMPRINTED AND EXPRESSED ALLELES OF THE INSULIN-LIKE GROWTH-FACTOR-2 GENE DURING BETA-CELL TUMORIGENESIS [J].