Surface design of SPR-based immunosensor for the effective binding of antigen or antibody in the evanescent field using mixed polymer matrix

被引：37

作者：

Toyama, S

Shoji, A

Yoshida, Y

Yamauchi, S

Ikariyama, Y

机构：

[1] Natl Rehabil Ctr Disabled, Res Inst, Tokorozawa, Saitama 3598555, Japan

[2] Toyo Univ, Fac Engn, Dept Appl Chem, Kawagoe, Saitama 3500815, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1998年 / 52卷 / 1-2期

关键词：

surface plasmon resonance; SPR; immunosensor; polymer matrix; hydroxyethyl starch; dextran sulfate;

D O I：

10.1016/S0925-4005(98)00257-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hydroxyethyl starch (HES) and dextran sulfate (DS), having respective molecular weight of 258 000 and 12 100 thousand Dalton, were oxidized and then immobilized at the surface of a gold thin layer for a surface plasmon resonance (SPR) sensor. Human serum albumin (HSA) was immobilized at the activated polymer matrix and then the sensor worked as a sensing device for anti-human serum albumin (anti-HSA). The sensor exhibited the maximum response when the mixing ratio of HES to DS was 3:2 (w:w). The detection limit was down to 10(-7) g ml(-1) at this mixing ratio. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：65 / 71

页数：7

共 11 条

[1] SURFACE-PLASMON RESONANCE AND IMMUNOSENSORS [J].