REAL-TIME BIOSPECIFIC INTERACTION ANALYSIS

The principles of instrumentation based on surface plasmon resonance (SPR) for real time biospecific interaction analysis are reviewed. A short description is given of the SPR principle and of a sensing chip utilizing a hydrogel as an interaction matrix. The focus of the contribution is, however, on the use of biospecific interaction analysis to obtain detailed information about the properties of biomolecules such as binding patterns, affinities and kinetic constants. In the context of interfacing biology with electronics the paper shows how interactions between biomolecules are transformed into the position of a dark spot on a photodiode array.