Transposon-based strategies for microbial functional genomics and proteomics

被引：121

作者：

Hayes, F ^{[1
]}

机构：

[1] Univ Manchester, Inst Sci & Technol, Dept Biomol Sci, Manchester M60 1QD, Lancs, England

来源：

ANNUAL REVIEW OF GENETICS | 2003年 / 37卷

关键词：

transposon; mutagenesis; bacteria; pentapeptide scanning; signature-tagged mutagenesis;

D O I：

10.1146/annurev.genet.37.110801.142807

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Transposons are mobile genetic elements that can relocate from one genomic location to another. As well as modulating gene expression and contributing to genome plasticity and evolution, transposons are remarkably diverse molecular tools for both whole-genome and single-gene studies in bacteria, yeast, and other microorganisms. Efficient but simple in vitro transposition reactions now allow the mutational analysis of previously recalcitrant microorganisms. Transposon-based signature tagged mutagenesis and genetic footprinting strategies have pinpointed essential genes and genes that are crucial for the infectivity of a variety of human and other pathogens. Individual proteins and protein complexes can be dissected by transposon-mediated scanning linker mutagenesis. These and other transposon-based approaches have reaffirmed the usefulness of these elements as simple yet highly effective mutagens for both functional genomic and proteomic studies of microorganisms.

引用

页码：3 / 29

页数：27

共 161 条

[81] 5 LISTERIA-MONOCYTOGENES GENES PREFERENTIALLY EXPRESSED IN INFECTED MAMMALIAN-CELLS - PLCA, PURH, PURD, PYRE AND AN ARGININE ABC TRANSPORTER GENE, ARPJ [J].

KLARSFELD, AD ;

GOOSSENS, PL ;

COSSART, P .

MOLECULAR MICROBIOLOGY, 1994, 13 (04) :585-597

[82]

Kleckner N, 1996, CURR TOP MICROBIOL, V204, P49

[83] Adaptation of signature-tagged mutagenesis for Toxoplasma gondii:: a negative screening strategy to isolate genes that are essential in restrictive growth conditions [J].

Knoll, LJ ;

Furie, GL ;

Boothroyd, JC .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 116 (01) :11-16

[84] Shufflons: Multiple inversion systems and integrons [J].

Komano, T .

ANNUAL REVIEW OF GENETICS, 1999, 33 :171-191

[85] Subcellular localization of the yeast proteome [J].

Kumar, A ;

Agarwal, S ;

Heyman, JA ;

Matson, S ;

Heidtman, M ;

Piccirillo, S ;

Umansky, L ;

Drawid, A ;

Jansen, R ;

Liu, Y ;

Cheung, KH ;

Miller, P ;

Gerstein, M ;

Roeder, GS ;

Snyder, M .

GENES & DEVELOPMENT, 2002, 16 (06) :707-719

[86] An integrated approach for finding overlooked genes in yeast [J].

Kumar, A ;

Harrison, PM ;

Cheung, KH ;

Lan, N ;

Echols, N ;

Bertone, P ;

Miller, P ;

Gerstein, MB ;

Snyder, M .

NATURE BIOTECHNOLOGY, 2002, 20 (01) :58-63

[87] High-throughput methods for the large-scale analysis of gene function by transposon tagging [J].

Kumar, A ;

des Etages, SA ;

Coelho, PSR ;

Roeder, GS ;

Snyder, M .

APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PT C: PROTEIN-PROTEIN INTERACTIONS AND GENOMICS, 2000, 328 :550-574

[88] Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage Mu [J].

Lamberg, A ;

Nieminen, S ;

Qiao, MQ ;

Savilahti, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (02) :705-712

[89] Fructose uptake in Sinorhizobium meliloti is mediated by a high-affinity ATP-binding cassette transport system [J].

Lambert, A ;

Osterås, M ;

Mandon, K ;

Poggi, MC ;

Le Rudulier, D .

JOURNAL OF BACTERIOLOGY, 2001, 183 (16) :4709-4717

[90] Purified mariner transposase is sufficient to mediate transposition in vitro [J].

Lampe, DJ ;

Churchill, MEA ;

Robertson, HM .

EMBO JOURNAL, 1996, 15 (19) :5470-5479

← 4 5 6 7 8 9 10 11 12 13 →