Multilayered peptide incorporated collagen tubules for peripheral nerve repair

被引:47
作者
Ahmed, MR [1 ]
Venkateshwarlu, U [1 ]
Jayakumar, R [1 ]
机构
[1] Cent Leather Res Inst, Bioorgan & Neurochem Lab, Madras 600020, Tamil Nadu, India
关键词
collagen; peripheral nerve regeneration; crosslinking; contact guidance; irradiation; tubule permeability;
D O I
10.1016/j.biomaterials.2003.09.075
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Successful nerve regeneration process was achieved with improved mechanical strength by crosslinking tubular nerve guides made up of collagen. The multilayered collagen sheets were prepared from laminar evaporation of collagen solution. Scanning electron micrograph of the collagen tubes crosslinked with glutaraldehyde (GTA), microwave irradiation showed porous, fibrillar structures of collagen filaments in these matrices. The mechanical property of the crosslinked collagen tubes was carried out by tensile strength measurements. Fourier transform infrared spectra of the collagen films show that the native triple helicity was unaltered during multilayered preparation. It was observed that the structural integrity is unaltered during the multilayer preparation. Microscopic analysis indicates that the tubule surface acts as a surface of adherence and proliferation for the sprouting axons from the cut proximal nerve stumps. Solute diffusion studies on these tubes indicate that they are highly porous to wide range of molecular sizes during regeneration. Among the two types of crosslinking, the microwave irradiated collagen conduits results in ample myelinated axons compared with GTA group, where we observed more unmyelinated axons. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2585 / 2594
页数:10
相关论文
共 44 条
[1]   THE MORPHOLOGY OF REGENERATING PERIPHERAL-NERVES IS MODULATED BY THE SURFACE MICROGEOMETRY OF POLYMERIC GUIDANCE CHANNELS [J].
AEBISCHER, P ;
GUENARD, V ;
VALENTINI, RF .
BRAIN RESEARCH, 1990, 531 (1-2) :211-218
[2]   Effectiveness of a bioabsorbable conduit in the repair of peripheral nerve [J].
Aldini, NN ;
Perego, G ;
Cella, GD ;
Maltarello, MC ;
Fini, M ;
Rocca, M ;
Giardino, R .
BIOMATERIALS, 1996, 17 (10) :959-962
[3]  
[Anonymous], 1984, SOLID PHASE PEPTIDE, P114
[4]  
BELLAMKONDA R, 1995, BIOMEDICAL ENG HDB, P1754
[5]  
BODANSZKY M, 1984, PRINCIPLES PEPTIDE S, P21
[6]   FLUOROMETRIC ASSAY OF PROTEINS IN NANOGRAM RANGE [J].
BOHLEN, P ;
STEIN, S ;
DAIRMAN, W ;
UDENFRIEND, S .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 155 (01) :213-220
[7]   Magnetically aligned collagen gel filling a collagen nerve guide improves peripheral nerve regeneration [J].
Ceballos, D ;
Navarro, X ;
Dubey, N ;
Wendelschafer-Crabb, G ;
Kennedy, WR ;
Tranquillo, RT .
EXPERIMENTAL NEUROLOGY, 1999, 158 (02) :290-300
[8]  
Chamberlain LJ, 2000, J COMP NEUROL, V417, P415, DOI 10.1002/(SICI)1096-9861(20000221)417:4<415::AID-CNE3>3.0.CO
[9]  
2-9
[10]  
Chamberlain LJ, 2000, J NEUROSCI RES, V60, P666, DOI 10.1002/(SICI)1097-4547(20000601)60:5<666::AID-JNR12>3.3.CO