Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction

被引：460

作者：

Zhang, YQ

Xue, CH ^{[1
]}

Xue, Y

Gao, RC

Zhang, XL

机构：

[1] Ocean Univ China, Dept Food Sci & Engn, Qingdao 266003, Peoples R China

[2] Qingdao Univ Sci & Technol, Dept Biol & Pharmaceut Engn, Qingdao 266042, Peoples R China

[3] Ocean Univ China, Marine Drug & Food Inst, Qingdao 266003, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2005年 / 340卷 / 11期

关键词：

degree of deacetylation; chitin; chitosan; X-ray powder diffraction;

D O I：

10.1016/j.carres.2005.05.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A new method to determine the degree of deacetylation (DD) of alpha-chitin and chitosan in the range of 17-94% DD using X-ray powder diffraction (XRD) is proposed. The results were calibrated using H-1 NMR spectroscopy for chitosan and FTIR for chitin, in comparison with the potentiometric titration method. The results showed a good linear correlation between the CrI020 from XRD and the calibrated DD value. This method is found to be simple, rapid and nondestructive to the sample. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1914 / 1917

页数：4

共 22 条

[1]

STUDIES ON CHITOSAN .1. DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN BY ULTRAVIOLET SPECTROPHOTOMETRY AND GEL-PERMEATION CHROMATOGRAPHY [J].