Simultaneous measurement of antibodies to three HIV-1 antigens in newborn dried blood-spot specimens using a multiplexed microsphere-based immunoassay

被引：62

作者：

Bellisario, R ^{[1
]}

Colinas, RJ

Pass, KA

机构：

[1] New York State Dept Hlth, Wadsworth Ctr, Div Genet Disorders, Albany, NY 12201 USA

[2] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Rensselaer, NY 12144 USA

来源：

EARLY HUMAN DEVELOPMENT | 2001年 / 64卷 / 01期

关键词：

newborn screening; HIV; microsphere; fluorescent immunoassay;

D O I：

10.1016/S0378-3782(01)00167-0

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

We developed a fluorescent immunoassay to simultaneously measure antibodies to three HIV-1 antigens: from newborn dried blood-spot specimens. The multiplexed assay uses fluorescent microspheres and a flow analyzer. The procedure is sensitive, precise and accurate, and can be expanded to simultaneously measure additional multiple analytes from a single specimen. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：21 / 25

页数：5

共 8 条

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