Ochratoxin A in sultanas from Turkey I: Survey of unprocessed sultanas from vineyards and packing-houses

被引:48
作者
Meyvaci, KB [1 ]
Altindisli, A
Aksoy, U
Eltem, R
Turgut, H
Arasiler, Z
Kartal, N
机构
[1] Ege Univ, Fac Agr, Dept Hort, TR-35100 Izmir, Turkey
[2] Ege Univ, Fac Engn, Dept Bioengn, TR-35100 Izmir, Turkey
[3] Ege Univ, TARIS Union Farmers Sales Cooperat, Ctr Res & Dev, TR-35100 Izmir, Turkey
来源
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2005年 / 22卷 / 11期
关键词
Ochratoxin A; high-performance liquid chromatography (HPLC); dried vine fruits; unprocessed sultanas; mycotoxin;
D O I
10.1080/02652030500199132
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A method for the determination of ochratoxin A ( OTA) in sultanas from Turkey using extraction with a sodium bicarbonate solution ( 2% NaHCO3) followed by immunoaffinity clean-up and liquid chromatography with fluorescence detection was used to assess the frequency of occurrence and level of OTA. In-house validation was carried out with spiked samples at levels of 0.15, 1.5, 5.0 and 10 mu g kg(-1) and average recoveries were 91, 93, 87 and 89%, respectively. The limits of detection and limit of quantification in Turkish sultanas were 0.026 and 0.09 mu g kg(-1), respectively. A survey for the presence of OTA was carried out on 264 unprocessed sultana samples during the production seasons between 1998 and 2000 collected annually from vineyards and from packing-houses. The analyses of unprocessed sultanas showed that 32.2% of the total number of samples contained no detectable OTA, whereas 9.8% of sultana samples had OTA concentrations above 10 mu g kg(-1), and the remaining 58% had levels within the range 0.026-10 mu g kg(-1). There were big differences in median concentrations between years. Considering the year of production, it appears that sultanas produced in 1998 and 2000 showed the lowest incidence of OTA contamination ( median < 0.02 mu g kg(-1)), whereas 2002 showed the highest incidence ( median 4.3 mu g kg(-1)1). The overall mean OTA concentration was calculated as 3.4 mu g kg(-1), and the overall median as 0.9 mu g kg(-1). Among the samples analysed, the highest detected level of OTA was 54 mu g kg(-1).
引用
收藏
页码:1138 / 1143
页数:6
相关论文
共 15 条
[11]   Determination of ochratoxin A in wine grapes:: comparison of extraction procedures and method validation [J].
Serra, R ;
Mendonça, C ;
Abrunhosa, L ;
Pietri, A ;
Venâncio, A .
ANALYTICA CHIMICA ACTA, 2004, 513 (01) :41-47
[12]   Ochratoxin A concentrations in Greek domestic wines and dried vine fruits [J].
Stefanaki, I ;
Foufa, E ;
Tsatsou-Dritsa, A ;
Dais, P .
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2003, 20 (01) :74-83
[13]   OCHRATOXIN A A TOXIC METABOLITE PRODUCED BY ASPERGILLUS OCHRACEUS WILH [J].
VANDERME.KJ ;
STEYN, PS ;
FOURIE, L ;
SCOTT, DB ;
THERON, JJ .
NATURE, 1965, 205 (4976) :1112-+
[14]   Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography [J].
Visconti, A ;
Pascale, M ;
Centonze, G .
JOURNAL OF CHROMATOGRAPHY A, 1999, 864 (01) :89-101
[15]  
vonderStegen G, 1997, FOOD ADDIT CONTAM, V14, P211, DOI 10.1080/02652039709374518