Biofilm formation by a fimbriae-deficient mutant of Actinobacillus actinomycetemcomitans

被引:37
作者
Inoue, T
Shingaki, R
Sogawa, N
Sogawa, CA
Asaumi, J
Kokeguchi, S
Fukui, K
机构
[1] Okayama Univ, Grad Sch Med & Dent, Dept Oral Microbiol, Okayama 7008525, Japan
[2] Okayama Univ, Grad Sch Med & Dent, Dept Dent Pharmacol, Okayama 7008525, Japan
[3] Okayama Univ, Grad Sch Med & Dent, Dept Oral & Maxillofacial Radiol, Okayama 7008525, Japan
关键词
biofilm; adherence; microcolony formation; fimbriae;
D O I
10.1111/j.1348-0421.2003.tb03454.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Actinobacillus actinomycetemcomitans strain 310-TR produces fimbriae and forms a tight biofilm in broth cultures, without turbid growth. The fimbriae-deficient mutant 310-DF, constructed in this study, was grown as a relatively fragile biofilm at the bottom of a culture vessel. Scanning electron microscopy revealed that on glass coverslips, 310-TR formed tight and spherical microcolonies, while 310-DF produced looser ones. These findings suggest that fimbriae are not essential for the surface-adherent growth but are required for enhancing cell-to-surface and cell-to-cell interactions to stabilize the biofilm. Treatment of the 310-DF biofilm with either sodium metaperiodate or DNase resulted in significant desorption of cells from glass surfaces, indicating that both carbohydrate residues and DNA molecules present on the cell surface are also involved in the biofilm formation.
引用
收藏
页码:877 / 881
页数:5
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