Prion gene sequence variation within diverse groups of US sheep, beef cattle, and deer

被引:84
作者
Heaton, MP
Leymaster, KA
Freking, BA
Hawk, DA
Smith, TPL
Keele, JW
Snelling, WM
Fox, JM
Chitko-McKown, CG
Laegreid, WW
机构
[1] USDA, ARS, MARC, Clay Ctr, Clay Ctr, NE 68933 USA
[2] Wyoming Game & Fish Dept, Laramie, WY 82071 USA
关键词
D O I
10.1007/s00335-003-2283-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prions are proteins that play a central role in transmissible spongiform encephalopathies in a variety of mammals. Among the most notable prion disorders in ungulates are scrapie in sheep, bovine spongiform encephalopathy in cattle, and chronic wasting disease in deer. Single nucleotide polymorphisms in the sheep prion gene (PRNP) have been correlated with susceptibility to natural scrapie in some populations. Similar correlations have not been reported in cattle or deer; however, characterization of PRNP nucleotide diversity in those species is incomplete. This report describes nucleotide sequence variation and frequency estimates for the PRNP locus within diverse groups of U.S. sheep, U.S. beef cattle, and free-ranging deer (Odocoileus virginianus and O. hemionus from Wyoming). DNA segments corresponding to the complete prion coding sequence and a 596-bp portion of the PRNP promoter region were amplified and sequenced from DNA panels with 90 sheep, 96 cattle, and 94 deer. Each panel was designed to contain the most diverse germplasm available from their respective populations to facilitate polymorphism detection. Sequence comparisons identified a total of 86 polymorphisms. Previously unreported polymorphisms were identified in sheep (9), cattle (13), and deer (32). The number of individuals sampled within each population was sufficient to detect more than 95% of all alleles present at a frequency greater than 0.02. The estimation of PRNP allele and genotype frequencies within these diverse groups of sheep, cattle, and deer provides a framework for designing accurate genotype assays for use in genetic epidemiology, allele management, and disease control.
引用
收藏
页码:765 / 777
页数:13
相关论文
共 47 条
[1]   Scrapie epidemic in a fully PrP-genotyped sheep flock [J].
Baylis, M ;
Goldmann, W ;
Houston, F ;
Cairns, D ;
Chong, A ;
Ross, A ;
Smith, A ;
Hunter, N ;
McLean, AR .
JOURNAL OF GENERAL VIROLOGY, 2002, 83 :2907-2914
[2]  
BELT PBG, 1996, BOVINE SPONGIFORM EN, P294
[3]   IDENTIFICATION OF 5 ALLELIC VARIANTS OF THE SHEEP PRP GENE AND THEIR ASSOCIATION WITH NATURAL SCRAPIE [J].
BELT, PBGM ;
MUILEMAN, IH ;
SCHREUDER, BEC ;
BOSDERUIJTER, J ;
GIELKENS, ALJ ;
SMITS, MA .
JOURNAL OF GENERAL VIROLOGY, 1995, 76 :509-517
[4]   Prion protein gene polymorphisms in natural goat scrapie [J].
Billinis, C ;
Panagiotidis, CH ;
Psychas, V ;
Argyroudis, S ;
Nicolaou, A ;
Leontides, S ;
Papadopoulos, O ;
Sklaviadis, T .
JOURNAL OF GENERAL VIROLOGY, 2002, 83 :713-721
[5]   PrP genotype contributes to determining survival times of sheep with natural scrapie [J].
Bossers, A ;
Schreuder, BEC ;
Muileman, IH ;
Belt, PBGM ;
Smits, MA .
JOURNAL OF GENERAL VIROLOGY, 1996, 77 :2669-2673
[6]   Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].
Ewing, B ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :186-194
[7]   Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment [J].
Ewing, B ;
Hillier, L ;
Wendl, MC ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :175-185
[8]   Identification of the single base change causing the callipyge muscle hypertrophy phenotype, the only known example of polar overdominance in mammals [J].
Freking, BA ;
Murphy, SK ;
Wylie, AA ;
Rhodes, SJ ;
Keele, JW ;
Leymaster, KA ;
Jirtle, RL ;
Smith, TPL .
GENOME RESEARCH, 2002, 12 (10) :1496-1506
[9]   DIFFERENT SCRAPIE-ASSOCIATED FIBRIL PROTEINS (PRP) ARE ENCODED BY LINES OF SHEEP SELECTED FOR DIFFERENT ALLELES OF THE SIP GENE [J].
GOLDMANN, W ;
HUNTER, N ;
BENSON, G ;
FOSTER, JD ;
HOPE, J .
JOURNAL OF GENERAL VIROLOGY, 1991, 72 :2411-2417
[10]   2 ALLELES OF A NEURAL PROTEIN GENE LINKED TO SCRAPIE IN SHEEP [J].
GOLDMANN, W ;
HUNTER, N ;
FOSTER, JD ;
SALBAUM, JM ;
BEYREUTHER, K ;
HOPE, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2476-2480