Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

被引：1048

作者：

Verma, Ayush ^{[1
]}

Uzun, Oktay ^{[1
]}

Hu, Yuhua ^{[2
]}

Hu, Ying ^{[1
]}

Han, Hee-Sun ^{[3
]}

Watson, Nicki ^{[4
]}

Chen, Suelin ^{[1
]}

Irvine, Darrell J. ^{[1
,5
]}

Stellacci, Francesco ^{[1
]}

机构：

[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[3] MIT, Dept Chem, Cambridge, MA 02139 USA

[4] MIT, Dept Biol, Cambridge, MA 02139 USA

[5] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

NATURE MATERIALS | 2008年 / 7卷 / 07期

关键词：

D O I：

10.1038/nmat2202

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic material of comparable size has shown this property yet. Cationic nano-objects pass through cell membranes by generating transient holes, a process associated with cytotoxicity. Studies aimed at generating cell-penetrating nanomaterials have focused on the effect of size, shape and composition. Here, we compare membrane penetration by two nanoparticle 'isomers' with similar composition (same hydrophobic content), one coated with subnanometre striations of alternating anionic and hydrophobic groups, and the other coated with the same moieties but in a random distribution. We show that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes. Our results offer a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio- and macro-molecules.

引用

页码：588 / 595

页数：8

共 45 条

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