Immobilized-metal affinity sorbents based on hydrophilic methacrylate polymers and their interaction with immunoglobulins

被引：15

作者：

Mézárosová, K ^{[1
]}

Tishchenko, G ^{[1
]}

Bouchal, K ^{[1
]}

Bleha, M ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Macromol Chem, Prague 16206, Czech Republic

来源：

REACTIVE & FUNCTIONAL POLYMERS | 2003年 / 56卷 / 01期

关键词：

poly[2-(2-hydroxyethoxy)ethyl methacrylate-co-ethylene dimethacrylate; ethylenediaminetriacetic acid; quinolin-8-ol; N-(2-pyridylmethyl)glycine; IMA sorbents;

D O I：

10.1016/S1381-5148(03)00029-4

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Hydrophilic homogeneous poly [2-(2-hydroxyethoxy)ethyl methacrylate-co-ethylene dimethacrylate] poly(DEGMA-co-EDMA) beads have been prepared as precursors for introducing the chelating groupings of ethylenediaminetriacetic acid, quinolin-8-ol, or N-(2-pyridylmethyl)glycine. The effect of the chelating ligand structure on coordination with Ni2+ ions and that of the metal complexes on binding the model protein (horseradish-peroxidase-specific immunoglobulin IgG(1)) was analyzed. It was found that the protein binding affinity and selectivity was the highest for IMA sorbents with quinolin-8-ol groups. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：27 / 35

页数：9

共 24 条

[11] MECHANISM OF 3-DIMENSIONAL POLYMERIZATION OF GLYCOL METHACRYLATES .3. CONTRIBUTION TO POLYMERIZATION KINETICS OF SYSTEM DIGLYCOL MONOMETHACRYLATE-GLYCOL DIMETHACRYLATES-WATER [J].

KOPECEK, J .

COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1971, 36 (07) :2703-&

[12] TITRIMETRY IN GLACIAL ACETIC ACID [J].

MARKUNAS, PC ;

RIDDICK, JA .

ANALYTICAL CHEMISTRY, 1951, 23 (02) :337-339

[13]

MUELLER KF, 1980, Patent No. 4224427

[14] Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins -: Evaluation of two anti-His-tag monoclonal antibodies [J].

Müller, KM ;

Arndt, KM ;

Bauer, K ;

Plückthun, A .

ANALYTICAL BIOCHEMISTRY, 1998, 259 (01) :54-61

[15] HARD AND SOFT ACIDS AND BASES - THE EVOLUTION OF A CHEMICAL CONCEPT [J].

PEARSON, RG .

COORDINATION CHEMISTRY REVIEWS, 1990, 100 :403-425

[16] METAL CHELATE AFFINITY CHROMATOGRAPHY, A NEW APPROACH TO PROTEIN FRACTIONATION [J].

PORATH, J ;

CARLSSON, J ;

OLSSON, I ;

BELFRAGE, G .

NATURE, 1975, 258 (5536) :598-599

[17] IMMOBILIZED METAL-ION AFFINITY ADSORPTION AND IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY OF BIOMATERIALS - SERUM-PROTEIN AFFINITIES FOR GEL-IMMOBILIZED IRON AND NICKEL IONS [J].

PORATH, J ;

OLIN, B .

BIOCHEMISTRY, 1983, 22 (07) :1621-1630

[18] IMPROVED PROCEDURE FOR DETERMINATION OF ALDEHYDES [J].

SIGGIA, S ;

MAXCY, W .

ANALYTICAL CHEMISTRY, 1947, 19 (12) :1023-1025

[19]

TIDWEL TT, 1990, SYNTHESIS-STUTTGART, V10, P860

[20]

Warburg O, 1942, BIOCHEM Z, V310, P384

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