Glutamate enhances DNA fragmentation in cultured spinal motor neurons of rat

被引：5

作者：

Manabe, Y ^{[1
]}

Wang, JM ^{[1
]}

Warita, H ^{[1
]}

Shiro, Y ^{[1
]}

Kashihara, K ^{[1
]}

Abe, K ^{[1
]}

机构：

[1] Okayama Univ, Sch Med, Dept Neurol, Okayama 7008558, Japan

来源：

NEUROLOGICAL RESEARCH | 2001年 / 23卷 / 01期

关键词：

glutamate; DNA fragmentation; spinal cord motor neurons; amyotrophic lateral sclerosis;

D O I：

10.1179/016164101101198163

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The role of glutamate in the mechanism of spinal motor neuron death is not fully understood. With addition of glutamate to primary culture of 11-day-old rat spinal cord, terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) positive nuclei were found in spinal large motor neurons from 24 h, and the number of TUNEL positive large motor neurons greatly increased at 48 h. In contrast, only a small number of large motor neurons became TUNEL positive at 48 h with addition of vehicle to the primary spinal cord culture. The present results show that excessive amount of glutamate enhances DNA fragmentation in developing large motor neuron of cultured spinal cord by involving in apoptotic process of the neurons.

引用

页码：79 / 82

页数：4

共 20 条

[1] APOPTOSIS AND NECROSIS - 2 DISTINCT EVENTS INDUCED, RESPECTIVELY, BY MILD AND INTENSE INSULTS WITH N-METHYL-D-ASPARTATE OR NITRIC-OXIDE SUPEROXIDE IN CORTICAL CELL-CULTURES [J].

BONFOCO, E ;

KRAINC, D ;

ANKARCRONA, M ;

NICOTERA, P ;

LIPTON, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7162-7166

[2] OXIDATIVE STRESS, GLUTAMATE, AND NEURODEGENERATIVE DISORDERS [J].

COYLE, JT ;

PUTTFARCKEN, P .

SCIENCE, 1993, 262 (5134) :689-695

[3] GLUTAMATE-INDUCED NEURONAL DEATH IS NOT A PROGRAMMED CELL-DEATH IN CEREBELLAR CULTURE [J].

DESSI, F ;

CHARRIAUTMARLANGUE, C ;

KHRESTCHATISKY, M ;

BENARI, Y .

JOURNAL OF NEUROCHEMISTRY, 1993, 60 (05) :1953-1955

[4] IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].

GAVRIELI, Y ;

SHERMAN, Y ;

BENSASSON, SA .

JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501

[5] Analysis of chromosome 5q13 genes in amyotrophic lateral sclerosis: Homozygous NAIP deletion in a sporadic case [J].

Jackson, M ;

Morrison, KE ;

AlChalabi, A ;

Bakker, M ;

Leigh, PN .

ANNALS OF NEUROLOGY, 1996, 39 (06) :796-800

[6] GLUTAMATE TRIGGERS INTERNUCLEOSOMAL DNA CLEAVAGE IN NEURONAL CELLS [J].

KURE, S ;

TOMINAGA, T ;

YOSHIMOTO, T ;

TADA, K ;

NARISAWA, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (01) :39-45

[7]

Larm JA, 1997, J NEUROCHEM, V69, P617

[8] Preincubation with protein synthesis inhibitors protects cortical neurons against oxygen-glucose deprivation-induced death [J].

Lobner, D ;

Choi, DW .

NEUROSCIENCE, 1996, 72 (02) :335-341

[9] APOPTOSIS IS INDUCED BY BETA-AMYLOID IN CULTURED CENTRAL-NERVOUS-SYSTEM NEURONS [J].

LOO, DT ;

COPANI, A ;

PIKE, CJ ;

WHITTEMORE, ER ;

WALENCEWICZ, AJ ;

COTMAN, CW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7951-7955

[10] APOPTOSIS IN SUBSTANTIA-NIGRA FOLLOWING DEVELOPMENTAL STRIATAL EXCITOTOXIC INJURY [J].

MACAYA, A ;

MUNELL, F ;

GUBITS, RM ;

BURKE, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :8117-8121

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