v-src oncogene-specific carboxy-terminal peptide is immunoprotective against Rous sarcoma growth in chickens with MHC class I allele B-F12

B(12) haplotype of the inbred chicken line CB (B(12)/B(12)) contains, like the bulk of chicken MHC(B) haplotypes, only a single dominantly expressed class I molecule (B-F). The peptide binding motifs for this major B-F12-molecule, in chickens of Rous sarcoma regressor line CB (B(12)/B(12)) have been determined. Using stringent and relaxed motifs, several peptides were found in the v-src molecule of the PR-RSV-C, but most of these peptides are identical with that of endogenous c-src. Only the v-src C-tail peptide(517-524) (LPACVLEV) contains critical anchor amino acids (valine at positions 5 and 8) and shows a sequence different from the corresponding c-src peptide. This v-src C-tail peptide up-regulates expression of the B-F12 class I molecule on PBL, as assessed by FACS analysis, and stimulates T cell proliferation in a [(3)H]thymidine uptake assay. A protective effect of the immune response to LPACVLEV against RSV challenge was demonstrated in CB (B(12)/B(12)) chickens immunised with peptides encapsulated in liposomes. (C) 2003 Elsevier Ltd. All rights reserved.