Enhanced cell penetration of acid-degradable particles functionalized with cell-penetrating peptides

被引：43

作者：

Cohen, Jessica L. ^{[1
]}

Almutairi, Adah ^{[1
]}

Cohen, Joel A. ^{[1
]}

Bernstein, Matt ^{[2
]}

Brody, Steven L. ^{[2
]}

Schuster, Daniel P. ^{[2
]}

Frechet, Jean M. J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Coll Chem, Berkeley, CA 94720 USA

[2] Washington Univ, Sch Med, St Louis, MO USA

来源：

BIOCONJUGATE CHEMISTRY | 2008年 / 19卷 / 04期

关键词：

D O I：

10.1021/bc700414j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biopharmaceuticals, such as proteins and DNA, have demonstrated their potential to prevent and cure diseases. The success of such therapeutic agents hinges upon their ability to cross complex barriers in the body and reach their target intact. In order to reap the full benefits of these therapeutic agents, a delivery vehicle capable of delivering cargo to all cell types, both phagocytic and non-phagocytic, is needed. This article presents the synthesis and evaluation of a microparticle delivery vehicle capable of cell penetration and sub-cellular triggered release of an encapsulated payload. pH-sensitive polyacrylamide particles functionalized with a polyarginine cell-penetrating peptide (CPP) were synthesized. The incorporation of a CPP into the microparticles led to efficient uptake by non-phagocytic cells in culture. In addition, the CPP-modified particles showed no cytotoxic effects at concentrations used in this study. The results suggest that these particles may provide a vehicle for the successful delivery of therapeutic agents to various cell types.

引用

页码：876 / 881

页数：6

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