Aromatic layered guanidines bind sequence-specifically to DNA minor groove with precise fit

被引:23
作者
Fukutomi, R
Tanatani, A
Kakuta, H
Tomioka, N
Itai, A
Hashimoto, Y
Shudo, K
Kagechika, H
机构
[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan
[3] Inst Med Mol Design, Bunkyo Ku, Tokyo 1130033, Japan
关键词
D O I
10.1016/S0040-4039(98)01378-1
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Tetraguanidines (1 and 2) with an aromatic multilayered structure exhibit potent binding to a specific site of DNAs with binding constants of the order of 10(7) M-1 as deduced from Scatchard analysis of ultrafiltration data and simulation of CD titration curves. NMR spectra of the complexes indicated that 1 and 2 bind DNAs sequence-specifically at the minor groove, as predicted by computational docking studies. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:6475 / 6478
页数:4
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