The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10

被引：135

作者：

Afkar, E

Lisak, J

Saltikov, C

Basu, P

Oremland, RS

Stolz, JF ^{[1
]}

机构：

[1] Duquesne Univ, Dept Biol Sci, Pittsburgh, PA 15282 USA

[2] CALTECH, Dept Geol & Planetary Sci, Pasadena, CA 91125 USA

[3] US Geol Survey, Menlo Pk, CA 94025 USA

[4] Duquesne Univ, Dept Chem & Biochem, Pittsburgh, PA 15282 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 226卷 / 01期

基金：

美国国家航空航天局;

关键词：

arsenate reductase; arsenate respiration; molybdoenzyme;

D O I：

10.1016/S0378-1097(03)00609-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The respiratory arsenate reductase from the Gram-positive, haloalkaliphile, Bacillus selenitireducens strain MLS10 was purified and characterized. It is a membrane bound heterodimer (150 kDa) composed of two subunits ArrA (110 kDa) and ArrB (34 kDa), with an apparent K-m for arsenate of 34 muM and V-max of 2.5 mumol min(-1) mg(-1). Optimal activity occurred at pH 9.5 and 150 g l(-1) of NaCl. Metal analysis (inductively coupled plasma mass spectrometry) of the holoenzyme and sequence analysis of the catalytic subunit (ArrA; the gene for which was cloned and sequenced) indicate it is a member of the DMSO reductase family of molybdoproteins. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：107 / 112

页数：6

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[1] MICROBE GROWS BY REDUCING ARSENIC [J].