CENTA as a chromogenic substrate for studying β-lactamases

被引：90

作者：

Bebrone, C

Moali, C

Mahy, F

Rival, S

Docquier, JD

Rossolini, GM

Fastrez, J

Pratt, RF

Frère, JM

Galleni, M ^{[1
]}

机构：

[1] Univ Liege, Inst Chim B6, Ctr Prot Engn, B-4000 Liege, Belgium

[2] Univ Catholique Louvain, Lab Biochim Phys & Biopolymeres, B-1348 Louvain, Belgium

[3] Univ Saarlandes, Fachrichtung Biochem 8 8, D-66123 Saarbrucken, Germany

[4] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy

[5] Wesleyan Univ, Dept Chem, Middletown, CT 06459 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 06期

关键词：

D O I：

10.1128/AAC.45.6.1868-1871.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CENTA, a chromogenic cephalosporin, is readily hydrolyzed by B-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of beta -lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.

引用

页码：1868 / 1871

页数：4

共 23 条

[11] Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-β-lactamase IMP-1 produced by Escherichia coli
Laraki, N
Franceschini, N
Rossolini, GM
Santucci, P
Meunier, C
de Pauw, E
Amicosante, G
Frère, JM
Galleni, M
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (04) : 902 - 906
[12] A COMPARATIVE-STUDY OF CLASS-D BETA-LACTAMASES
LEDENT, P
RAQUET, X
JORIS, B
VANBEEUMEN, J
FRERE, JM
[J]. BIOCHEMICAL JOURNAL, 1993, 292 : 555 - 562
[13] STREPTOMYCES K15 DD-PEPTIDASE-CATALYZED REACTIONS WITH SUICIDE BETA-LACTAM CARBONYL DONORS
LEYHBOUILLE, M
NGUYENDISTECHE, M
PIRLOT, S
VEITHEN, A
BOURGUIGNON, C
GHUYSEN, JM
[J]. BIOCHEMICAL JOURNAL, 1986, 235 (01) : 177 - 182
[14] NOVEL METHOD FOR DETECTION OF BETA-LACTAMASES BY USING A CHROMOGENIC CEPHALOSPORIN SUBSTRATE
OCALLAGHAN, CH
SHINGLER, AH
KIRBY, SM
MORRIS, A
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1972, 1 (04) : 283 - +
[15] Mono- and binuclear Zn-β-lactamase from Bacteroides fragilis:: catalytic and structural roles of the zinc ions
Paul-Soto, R
Hernandez-Valladares, M
Galleni, M
Bauer, R
Zeppezauer, M
Frère, JM
Adolph, HW
[J]. FEBS LETTERS, 1998, 438 (1-2) : 137 - 140
[16] TEM BETA-LACTAMASE MUTANTS HYDROLYZING THIRD-GENERATION CEPHALOSPORINS
RAQUET, X
LAMOTTEBRASSEUR, J
FONZE, E
GOUSSARD, S
COURVALIN, P
FRERE, JM
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 244 (05) : 625 - 639
[17] Characterization and sequence of the Chryseobacterium (Flavobacterium) meningosepticum carbapenemase:: a new molecular class B β-lactamase showing a broad substrate profile
Rossolini, GM
Franceschini, N
Riccio, ML
Mercuri, PS
Perilli, M
Galleni, M
Frere, JM
Amicosante, G
[J]. BIOCHEMICAL JOURNAL, 1998, 332 : 145 - 152
[18] X-ray analysis of the NMC-A β-lactamase at 1.64-Å resolution, a class A carbapenemase with broad substrate specificity
Swarén, P
Maveyraud, L
Raquet, X
Cabantous, S
Duez, C
Pédelacq, JD
Mariotte-Boyer, S
Mourey, L
Labia, R
Nicolas-Chanoine, MH
Nordmann, P
Frère, JM
Samama, JP
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (41) : 26714 - 26721
[19] Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability
Valladares, MH
Felici, A
Weber, G
Adolph, HW
Zeppezauer, M
Rossolini, GM
Amicosante, G
Frere, JM
Galleni, M
[J]. BIOCHEMISTRY, 1997, 36 (38) : 11534 - 11541
[20] SITE-DIRECTED MUTAGENESIS OF PROPOSED ACTIVE-SITE RESIDUES OF PENICILLIN-BINDING PROTEIN-5 FROM ESCHERICHIA-COLI
VANDERLINDEN, MPG
DEHAAN, L
DIDEBERG, O
KECK, W
[J]. BIOCHEMICAL JOURNAL, 1994, 303 : 357 - 362

← 1 2 3 →