CENTA as a chromogenic substrate for studying β-lactamases

被引:90
作者
Bebrone, C
Moali, C
Mahy, F
Rival, S
Docquier, JD
Rossolini, GM
Fastrez, J
Pratt, RF
Frère, JM
Galleni, M [1 ]
机构
[1] Univ Liege, Inst Chim B6, Ctr Prot Engn, B-4000 Liege, Belgium
[2] Univ Catholique Louvain, Lab Biochim Phys & Biopolymeres, B-1348 Louvain, Belgium
[3] Univ Saarlandes, Fachrichtung Biochem 8 8, D-66123 Saarbrucken, Germany
[4] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy
[5] Wesleyan Univ, Dept Chem, Middletown, CT 06459 USA
关键词
D O I
10.1128/AAC.45.6.1868-1871.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
CENTA, a chromogenic cephalosporin, is readily hydrolyzed by B-lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of beta -lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.
引用
收藏
页码:1868 / 1871
页数:4
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