Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg

被引:47
作者
He, XW
Wang, F
Jiang, L
Li, J
Liu, SK
Xiao, ZY
Jin, XQ
Zhang, YN
He, Y
Li, K
Guo, YJ
Sun, SH
机构
[1] Second Mil Med Univ, Dept Med Genet, Shanghai 200433, Peoples R China
[2] USN, Med Res Inst, Ctr New Drug Res, Shanghai 200433, Peoples R China
[3] Second Mil Med Univ, Sch Pharmaceut Sci, Shanghai 200433, Peoples R China
关键词
D O I
10.1099/vir.0.80575-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The purpose of this work was to assess the ability of plasmid DNA encoding hepatitis B virus (HBV) HBsAg encapsulated in poly(DL-lactide-co-glycolic acid) (PLGA) microparticles to induce local and systemic HBsAg-specific immunity following a single dose of oral immunization. RT-PCR analysis demonstrated prolonged transcription of plasmid DNA, consistent with the sustained expression and presentation of target antigen observed by confocal laser scanning microscopy, in gut-associated lymphocyte tissue (GALT) from mice immunized orally with plasmid DNA encapsulated into PLGA microparticles. Oral administration of PLGA-DNA microparticles induced a long-lasting and stable antigen-specific antibody response, both serum total antibody and intestinal IgA, in BALB/c mice. Mice immunized orally exhibited antigen-specific gamma interferon production and cytotoxic T lymphocyte responses in spleen and GALT after restimulation in vitro with HBsAg or tumour cells stably expressing HBsAg. In contrast, naked DNA vaccines given by intramuscular injection induced only systemic cellular and humoral responses to HBsAg, which were much lower than the responses elicited by oral DNA encapsulated in PLGA microparticles at equivalent doses. The results are encouraging with regard to obtaining good compliance and vaccination coverage with candidate plasmid DNA vaccines, especially in developing countries.
引用
收藏
页码:601 / 610
页数:10
相关论文
共 34 条
[1]   Single-dose mucosal immunization with biodegradable microparticles containing a Schistosoma mansoni antigen [J].
Baras, B ;
Benoit, MA ;
Dupré, L ;
Poulain-Godefroy, O ;
Schacht, AM ;
Capron, A ;
Gillard, J ;
Riveau, G .
INFECTION AND IMMUNITY, 1999, 67 (05) :2643-2648
[2]   Vaccination of seronegative volunteers with a human immunodeficiency virus type 1 env/rev DNA vaccine induces antigen-specific proliferation and lymphocyte production of β-chemokines [J].
Boyer, JD ;
Cohen, AD ;
Vogt, S ;
Schumann, K ;
Nath, B ;
Ahn, L ;
Lacy, K ;
Bagarazzi, ML ;
Higgins, TJ ;
Baine, Y ;
Ciccarelli, RB ;
Ginsberg, RS ;
MacGregor, RR ;
Weiner, DB .
JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (02) :476-483
[3]   DNA vaccine for hepatitis B: Evidence for immunogenicity in chimpanzees and comparison with other vaccines [J].
Davis, HL ;
McCluskie, MJ ;
Gerin, JL ;
Purcell, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (14) :7213-7218
[4]   Mucosal and systemic immune responses to a human immunodeficiency virus type 1 epitope induced upon vaginal infection with a recombinant influenza a virus [J].
Garulli, B ;
Kawaoka, Y ;
Castrucci, MR .
JOURNAL OF VIROLOGY, 2004, 78 (02) :1020-1025
[5]   Formulations containing poly(lactide-co-glycolide) and plasmid DNA expression vectors [J].
Hedley, ML .
EXPERT OPINION ON BIOLOGICAL THERAPY, 2003, 3 (06) :903-910
[6]   Immune responses and protection obtained by oral immunization with rotavirus VP4 and VP7 DNA vaccines encapsulated in microparticles [J].
Herrmann, JE ;
Chen, SC ;
Jones, DH ;
Tinsley-Bown, A ;
Fynan, EF ;
Greenberg, HB ;
Farrar, GH .
VIROLOGY, 1999, 259 (01) :148-153
[7]   Development of systems for targeting the regional lymph nodes for diagnostic imaging:: In vivo behaviour of colloidal PEG-coated magnetite nanospheres in the rat following interstitial administration [J].
Illum, L ;
Church, AE ;
Butterworth, MD ;
Arien, A ;
Whetstone, J ;
Davis, SS .
PHARMACEUTICAL RESEARCH, 2001, 18 (05) :640-645
[8]   Oral DNA vaccination promotes mucosal and systemic immune responses to HIV envelope glycoprotein [J].
Kaneko, H ;
Bednarek, I ;
Wierzbicki, A ;
Kiszka, I ;
Dmochowski, M ;
Wasik, TJ ;
Kaneko, Y ;
Kozbor, D .
VIROLOGY, 2000, 267 (01) :8-16
[9]   Induction of mucosal and systemic immune response by oral immunization with H-pylori lysates encapsulated in poly(D,L-lactide-co-glycolide) microparticles [J].
Kim, SY ;
Doh, HJ ;
Ahn, JS ;
Ha, YJ ;
Jang, MH ;
Chung, SI ;
Park, HJ .
VACCINE, 1999, 17 (06) :607-616
[10]  
Klencke B, 2002, CLIN CANCER RES, V8, P1028