Immobilization of lipases on clay, Celite 545, diethylaminoethyl-, and carboxymethyl-Sephadex and their interesterification activity

Lipases AK, PS (both from Pseudomonas so), and AY (from Candida rugosa) were immobilized on clay, Celite 545, DEAE-Sephadex, or CM-Sephadex and used to synthesize structured lipids from tricaprylin and trilinolein. Lipases AK and AY immobilized on Celite 545 had the highest interesterification activity (0.11 and 0.35 units, respectively) while lipase PS had the highest activity (0.18 units) when immobilized on CM-Sephadex. During 72 h reaction, the highest product yields were obtained in Celite AK (224 mu mol), and CM PS (290 mu mol) after 48 h and Celite AY (501 mu mol) after 24 h. Lipase AK immobilized on DEAE-Sephadex was the most stable for repeated (5 times) operation.