Chemical and biological characterization of pectin-like polysaccharides from the bark of the Malian medicinal tree Cola cordifolia

被引：66

作者：

Austarheim, Ingvild ^{[1
]}

Christensen, Bjorn E. ^{[2
]}

Hegna, Ida K. ^{[1
]}

Petersen, Bent O. ^{[3
]}

Duus, Jens O. ^{[3
]}

Bye, Ragnar ^{[1
]}

Michaelsen, Terje E. ^{[1
,4
]}

Diallo, Drissa ^{[5
]}

Inngjerdingen, Marit ^{[6
]}

Paulsen, Berit S. ^{[1
]}

机构：

[1] Univ Oslo, Sch Pharm, N-0316 Oslo, Norway

[2] NTNU, Dept Biotechnol, NOBIPOL, Trondheim, Norway

[3] Carlsberg Labs, Copenhagen, Denmark

[4] Natl Inst Res Publ Hlth, Oslo, Norway

[5] Dept Tradit Med, Bamako, Mali

[6] Univ Oslo, Fac Med, IMMI, N-0316 Oslo, Norway

来源：

CARBOHYDRATE POLYMERS | 2012年 / 89卷 / 01期

关键词：

Cola cordifolia; Pectin; Structure; Rhamnogalacturonan; Immunological activity; Complement system; Cytotoxicity; PLANTAGO-MAJOR L; LIGHT-SCATTERING; VISCOSITY DETECTORS; CHAIN STIFFNESS; ARABINOGALACTAN; SPECTROSCOPY; COMPLEMENT; EXTENSION; EXTRACTS; LEAVES;

D O I：

10.1016/j.carbpol.2012.03.005

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The bark of Cola cordifolia used in Malian traditional medicine contains unusual types of polysaccharides with immunomodulating activities. We report for the first time on the structure of a polymer designated CC1P1 having the repeating structure [2 ->)[alpha-D-Gal(1 -> 3)]alpha-L-Rha(1 -> 4)alpha-D-GalA(1 ->] as determined by NMR and GC/MS. alpha-Linked Gal is unusual in pectins. The M-w of 135 kDa was determined by SEC-MALLS. CC1P2 (1400 kDa), another polymer, having the same backbone, but this was substituted with alpha-4-OMe-GlcA, alpha-2-OMe-Gal and alpha-Gal as terminal units. CC1P1 shows a high complement-fixing activity. IC50 being 2.2 times lower than the positive pectin control PMII (IC50 appr. 71 mu g/mL) while IC50 of CC1P2 is 1.8 times lower. The simple structure of CC1P1 did not activate macrophages, while CC1P2 (100 mu g/mL) showed the same potency as the positive controls PMII (100 mu g/mL) and LPS (500 ng/mL). No cytotoxicity was detected. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：259 / 268

页数：10

共 39 条

[1]

C-13 NUCLEAR MAGNETIC-RESONANCE DATA FOR OLIGOSACCHARIDES [J].