Characterization of dynamic cellular adhesion of osteoblasts using atomic force microscopy

被引:39
作者
Simon, A
Cohen-Bouhacina, T
Porté, MC
Aimé, JP
Amédée, J
Bareille, R
Baquey, C
机构
[1] Univ Bordeaux 1, CPMOH, F-33405 Talence, France
[2] Univ Bordeaux 2, INSERM U443, F-33076 Bordeaux, France
来源
CYTOMETRY PART A | 2003年 / 54A卷 / 01期
关键词
cellular adhesion; osteoblasts; atomic force microscopy; cytoskeletal; elastic modulus;
D O I
10.1002/cyto.a.10052
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: Atomic force microscopy (AFM) can be used to visualize the cell morphology in an aqueous environment and in real time. It also allows the investigation of mechanical properties such as cell compliance as a function of cell attachment. This study characterized and evaluated osteoblast adhesion by AFM. Methods: Human bone marrow stromal cells were cultured on two types of surface to induce weak and strong cellular adhesions. Results: Cells were considered as spreading if they had a flattened and lengthened shape and a cytoskeletal organization in the submembrane cytosolic region. Cell detachment demonstrated different adhesion states between adherent cells to be distinguished. The stability of the cytoskeletal fibers indicated that cells were adherent. The elastic modulus was estimated by two complementary approaches. The values deduced were between 3 x 10(2) and 2 x 10(5) Nm(-2) according to the state of cell adhesion and the approaches used to measure this elastic modulus. Conclusions: Although the results were qualitative, a relation may be deduced between the elasticity of living cells as demonstrated by cytoskeletal organization and the state of cell adhesion. The technique could be used to determine the adhesion state of an adherent osteoblast observed under AFM. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:36 / 47
页数:12
相关论文
共 58 条
[11]   FOCAL CONTACTS - TRANSMEMBRANE LINKS BETWEEN THE EXTRACELLULAR-MATRIX AND THE CYTOSKELETON [J].
BURRIDGE, K ;
FATH, K .
BIOESSAYS, 1989, 10 (04) :104-108
[12]   IMAGING CELLS WITH THE ATOMIC FORCE MICROSCOPE [J].
BUTT, HJ ;
WOLFF, EK ;
GOULD, SAC ;
NORTHERN, BD ;
PETERSON, CM ;
HANSMA, PK .
JOURNAL OF STRUCTURAL BIOLOGY, 1990, 105 (1-3) :54-61
[13]  
CARPEAK RW, 1996, J VAC SCI TECHNOL, V4, P1289
[14]   Cellular control lies in the balance of forces [J].
Chicurel, ME ;
Chen, CS ;
Ingber, DE .
CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (02) :232-239
[15]   A NONDESTRUCTIVE METHOD FOR DETERMINING THE SPRING CONSTANT OF CANTILEVERS FOR SCANNING FORCE MICROSCOPY [J].
CLEVELAND, JP ;
MANNE, S ;
BOCEK, D ;
HANSMA, PK .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1993, 64 (02) :403-405
[16]   Measuring the elastic properties of thin polymer films with the atomic force microscope [J].
Domke, J ;
Radmacher, M .
LANGMUIR, 1998, 14 (12) :3320-3325
[17]   Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy [J].
Domke, J ;
Dannöhl, S ;
Parak, WJ ;
Müller, O ;
Aicher, WK ;
Radmacher, M .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2000, 19 (04) :367-379
[18]   RETENTION OF LEUKOCYTES IN CAPILLARIES - ROLE OF CELL-SIZE AND DEFORMABILITY [J].
DOWNEY, GP ;
DOHERTY, DE ;
SCHWAB, B ;
ELSON, EL ;
HENSON, PM ;
WORTHEN, GS .
JOURNAL OF APPLIED PHYSIOLOGY, 1990, 69 (05) :1767-1778
[19]   GRANULA MOTION AND MEMBRANE SPREADING DURING ACTIVATION OF HUMAN PLATELETS IMAGED BY ATOMIC-FORCE MICROSCOPY [J].
FRITZ, M ;
RADMACHER, M ;
GAUB, HE .
BIOPHYSICAL JOURNAL, 1994, 66 (05) :1328-1334
[20]   Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton [J].
Haga, H ;
Sasaki, S ;
Kawabata, K ;
Ito, E ;
Ushiki, T ;
Sambongi, T .
ULTRAMICROSCOPY, 2000, 82 (1-4) :253-258