Subunit-subunit interactions in the human 26S proteasome

被引：25

作者：

Chen, Chuan ^{[1
]}

Huang, Caoxin ^{[1
]}

Chen, Shouhui ^{[2
]}

Liang, Jie

Lin, Wenbo ^{[1
]}

Ke, Guifen ^{[1
]}

Zhang, Hongxin ^{[1
]}

Wang, Bing ^{[3
]}

Huang, Jian ^{[4
]}

Han, Zeguang ^{[4
]}

Ma, Lixin ^{[5
]}

Huo, Keke ^{[6
]}

Yang, Xiaoming

Yang, Pengyuan ^{[6
]}

He, Fuchu ^{[7
]}

Tao, Tao ^{[1
]}

机构：

[1] Xiamen Univ, Sch Life Sci, Key Lab Cell Biol & Tumor Cell Engn, Minist Educ, Xiamen, Peoples R China

[2] Zhongshan Hosp Xiamen, Xiamen, Peoples R China

[3] Med Sch Xiamen, Xiamen, Peoples R China

[4] Natl Ctr Genom, Shanghai, Peoples R China

[5] Hubei Univ, Wuhan, Peoples R China

[6] Fudan Univ, Shanghai 200433, Peoples R China

[7] Beijing Proteom Ctr, Beijing, Peoples R China

来源：

PROTEOMICS | 2008年 / 8卷 / 03期

关键词：

26S proteasome; interaction; subunit; yeast two-hybrid analysis;

D O I：

10.1002/pmic.200700588

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs in the human 26S proteasome. About 10% (11/114) of these interacting-pairs was confirmed by the GST-pull down analysis. Among these observed interacting subunits, 58% (66/114) are novel and the rest 42% (48/114) has been reported previously in human or in other species. We observed new interactions between the 19S regulatory particle and the P-rings of the 20S catalytic particle and therefore proposed a modified model of the 26S proteasome.

引用

页码：508 / 520

页数：13

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