学术探索
学术期刊
新闻热点
数据分析
智能评审

Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from human brain tissue

被引：42
作者：
Pennington, K
McGregor, E
Beasley, CL
Everall, I
Cotter, D
Dunn, MJ
机构：
[1] Kings Coll London, Dept Psychol Med, Sect Neuropathol & Psychiat, Inst Psychiat, London SE5, England
[2] Kings Coll London, Inst Psychiat, Dept Neurosci, London SE5, England
来源：
PROTEOMICS | 2004年 / 4卷 / 01期
关键词：
basic proteins; DeStreak; human brain; isoelectric focusing; two-dimensional gel electrophoresis;
D O I：
10.1002/pmic.200300624
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A major cause of poor resolution in the alkaline pH range of two-dimensional electrophoresis (2-DE) gels is unsatisfactory separation of basic proteins in the first dimension. We have compared methods for the separation of basic proteins in the isoelectric focusing dimension of human brain proteins. The combined use of anodic cup-loading and the hydroxyethyldisulphide containing solution (DeStreak) produced better resolution in both analytical and micropreparative protein loaded 2-DE gels than the other methods investigated.
引用
收藏
页码：27 / 30
页数：4
相关论文
共 12 条
[1]   ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS - PRINCIPLE, METHODOLOGY AND SOME APPLICATIONS [J].
BJELLQVIST, B ;
EK, K ;
RIGHETTI, PG ;
GIANAZZA, E ;
GORG, A ;
WESTERMEIER, R ;
POSTEL, W .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1982, 6 (04) :317-339
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST DIMENSION (IPG-DALT) - THE STATE-OF-THE-ART AND THE CONTROVERSY OF VERTICAL VERSUS HORIZONTAL SYSTEMS [J].
GORG, A ;
BOGUTH, G ;
OBERMAIER, C ;
POSCH, A ;
WEISS, W .
ELECTROPHORESIS, 1995, 16 (07) :1079-1086
[4]  
Hoving S, 2002, PROTEOMICS, V2, P127, DOI 10.1002/1615-9861(200202)2:2<127::AID-PROT127>3.0.CO
[5]  
2-Y
[6]  
Olsson I, 2002, PROTEOMICS, V2, P1630, DOI 10.1002/1615-9861(200211)2:11<1630::AID-PROT1630>3.0.CO
[7]  
2-N
[8]   SAMPLE APPLICATION BY IN-GEL REHYDRATION IMPROVES THE RESOLUTION OF 2-DIMENSIONAL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST-DIMENSION [J].
RABILLOUD, T ;
VALETTE, C ;
LAWRENCE, JJ .
ELECTROPHORESIS, 1994, 15 (12) :1552-1558
[9]   Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients [J].
Sanchez, JC ;
Rouge, V ;
Pisteur, M ;
Ravier, F ;
Tonella, L ;
Moosmayer, M ;
Wilkins, MR ;
Hochstrasser, DF .
ELECTROPHORESIS, 1997, 18 (3-4) :324-327
[10]   Hyperubiquitination of proteins in dilated cardiomyopathy [J].
Weekes, J ;
Morrison, K ;
Mullen, A ;
Wait, R ;
Barton, P ;
Dunn, MJ .
PROTEOMICS, 2003, 3 (02) :208-216
12