Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells

被引：40

作者：

Mizushima, T

Tsutsumi, S

Rokutan, K

Tsuchiya, T

机构：

[1] Okayama Univ, Fac Pharmaceut Sci, Okayama 7008530, Japan

[2] Univ Tokushima, Sch Med, Dept Nutr, Tokushima 7708503, Japan

来源：

DIGESTIVE DISEASES AND SCIENCES | 1999年 / 44卷 / 03期

关键词：

geranylgeranylacetone; gastric mucosal cell; apoptosis; heat-shock proteins;

D O I：

10.1023/A:1026692920848

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The purpose of this study was to elucidate the molecular mechanism of action of geranylgeranylacetone, an antiulcer drug. Treatment with ethanol for 8 hr at the optimum concentration (7.5%) caused apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells. Pretreatment of cells with geranylgeranylacetone suppressed the DNA fragmentation in a dose-dependent manner. The maximum effect was achieved at 10(-6) M, at which concentration the drug was previously shown to induce heat-shack proteins. The suppression required an incubation period longer than 1 hr. Pretreatment of cells with low concentrations of ethanol also prevented DNA fragmentation.

引用

页码：510 / 514

页数：5

共 20 条

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